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Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants

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Preeclampsia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1710))

Abstract

Ex vivo culture of human placental explants has long allowed placentologists to study the milieu of soluble factors secreted by the human placenta throughout gestation while retaining the correct three-dimensional structure of the placental villi. Here, we detail the placental explant culture method employed in our laboratory to collect extracellular vesicles which are known to be released by the human placenta throughout pregnancy from 6 weeks of gestation. Using this method, at least three different populations of placental extracellular vesicles can be simultaneously collected from each placental sample, allowing for comparative analysis of the cargos and downstream effects of the different types of extracellular vesicles produced by the human placenta.

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Acknowledgments

Mancy Tong is a recipient of the University of Auckland Health Research Doctoral Scholarship and the Freemasons Postgraduate Scholarship.

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Authors and Affiliations

  1. Department of Obstetrics and Gynaecology, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag, 92019, Auckland, New Zealand

    Mancy Tong & Lawrence W. Chamley

Authors
  1. Mancy Tong
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  2. Lawrence W. Chamley
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Correspondence to Lawrence W. Chamley .

Editor information

Editors and Affiliations

  1. Monash Medical Centre, Monash University, Clayton, Victoria, Australia

    Padma Murthi

  2. INRS-Institut Armand-Frappier, Laval, Québec, Canada

    Cathy Vaillancourt

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Tong, M., Chamley, L.W. (2018). Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7498-6_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7497-9

  • Online ISBN: 978-1-4939-7498-6

  • eBook Packages: Springer Protocols

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