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Preeclampsia

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Padma Murthi

    1. Monash Medical Centre, Monash University, Clayton, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Cathy Vaillancourt

    1. INRS-Institut Armand-Frappier, Laval, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1710)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Diagnostic Imaging: Ultrasound

    • Stefan C. Kane, Su Lynn Khong, Fabricio da Silva Costa
    Pages 1-8

  3. Biomarker Immunoassays in the Diagnosis of Preeclampsia: Calculating the sFlt1/PlGF Ratio Using the Cobas®e 411 Analyser

    • Carin Black, Fabricio da Silva Costa
    Pages 9-26

  4. Assessing the Circulating Placental-Specific Anti-angiogenic Protein sFLT-1 e15a in Preeclampsia

    • Kirsten Palmer
    Pages 27-37

  5. Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Preeclampsia

    • Sebastian R. Hobson, Rebecca Lim, Joanne C. Mockler, Seshini Gurusinghe, Euan M. Wallace
    Pages 39-52

  6. Genetic Approaches in Preeclampsia

    • Hannah E. J. Yong, Padma Murthi, Shaun P. Brennecke, Eric K. Moses
    Pages 53-72

  7. Epigenetics and Preeclampsia: Programming of Future Outcomes

    • Alberto Borges Peixoto, Liliam Cristine Rolo, Luciano Marcondes Machado Nardozza, Edward Araujo Júnior
    Pages 73-83

  8. Inflammatory and Immune System Markers

    • Kelly J. McKelvey, Gaayathri Ariyakumar, Sharon A. McCracken
    Pages 85-101

  9. Methods to Enrich Exosomes from Conditioned Media and Biological Fluids

    • Shayna Sharma, Katherin Scholz-Romero, Gregory E. Rice, Carlos Salomon
    Pages 103-115

  10. Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants

    • Mancy Tong, Lawrence W. Chamley
    Pages 117-129

  11. Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation

    • Andrew Lai, Omar Elfeky, Gregory E. Rice, Carlos Salomon
    Pages 131-138

  12. Proteomics Method to Identification of Protein Profiles in Exosomes

    • Andrew Lai, Vyjayanthi Kinhal, Zarin Nuzhat, Ramkumar Menon, Gregory E. Rice, Carlos Salomon
    Pages 139-153

  13. Harvesting and Characterization of Syncytial Nuclear Aggregates Following Culture of First Trimester Human Placental Explants

    • Priyadarshini Pantham, Lawrence W. Chamley
    Pages 155-163

  14. Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index

    • Severine A. Degrelle, Thierry Fournier
    Pages 165-171

  15. Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of Off-Target Effects

    • Paul Brownbill, Neil Sebire, Erin V. McGillick, Stacey Ellery, Padma Murthi
    Pages 173-189

  16. Immunohistological Techniques

    • Evangelina Capobianco, Nora Martinez
    Pages 191-201

  17. Using a Next-Generation Sequencing Approach to Profile MicroRNAs from Human Origin

    • Dominic Guanzon, Juvita Delancy Iljas, Gregory E. Rice, Carlos Salomon
    Pages 203-217

  18. Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta

    • Hélène Clabault, Laetitia Laurent, J. Thomas Sanderson, Cathy Vaillancourt
    Pages 219-231

  19. Analyzing Trophoblast Function Using Cell-Based Assays

    • Katie L. Powell, Anthony W. Ashton
    Pages 233-246

  20. Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and Decidua Basalis

    • Gina D. Kusuma, Mohamed H. Abumaree, Mark D. Pertile, Bill Kalionis
    Pages 247-266
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Keywords

About this book

This volume presents the latest developments and techniques used to study the physiopathology, diagnosis, and treatment of pre-eclampsia. Written for clinicians, obstetricians, basic scientists, researchers, and students the chapters in this book provide methods to study placental function using in vitro and ex vivo model systems, comprehensive genetic analysis of pre-eclampsia, identifying critical angiogenic factors associated with the development of pre-eclampsia, and controlled experiments that investigate potential therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls, as well as a description of the limitations and advantages of the described techniques.

Cutting-edge and comprehensive, Pre-Eclampsia: Methods andProtocols is a valuable resource for anyone interested in learning more about the critical role of the placenta in the maternal-fetal relationship.



Editors and Affiliations

  • Monash Medical Centre, Monash University, Clayton, Australia

    Padma Murthi

  • INRS-Institut Armand-Frappier, Laval, Canada

    Cathy Vaillancourt

Bibliographic Information

  • Book Title: Preeclampsia

  • Book Subtitle: Methods and Protocols

  • Editors: Padma Murthi, Cathy Vaillancourt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7498-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7497-9Published: 02 December 2017

  • Softcover ISBN: 978-1-4939-8504-3Published: 05 September 2018

  • eBook ISBN: 978-1-4939-7498-6Published: 30 November 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 355

  • Number of Illustrations: 13 b/w illustrations, 50 illustrations in colour

  • Topics: Molecular Medicine

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Health & Hospitals, Pharma

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