Abstract
During the last decade, multiple animal models have been developed to mimic hallmarks of pregnancy-induced hypertension (PIH) diseases, which include gestational hypertension, preeclampsia (PE), or eclampsia. Converging in vitro, ex vivo, and clinical studies from our group strongly suggested the potential involvement of the new angiogenic factor EG-VEGF (endocrine gland-derived-VEGF) in the development of PIH. Here, we described the protocol that served to demonstrate that maintenance of EG-VEGF production over 11.5 days post coitus (dpc) in the gravid mice caused the development of PIH. The developed model exhibited most hallmarks of preeclampsia.
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Acknowledgments
We thank Dr. J. Boutonnat and Dr. A. Florin for their help for the histological assessment of kidney sections. We acknowledge the following sources of funding: Institut National de la Santé et de la Recherche Médicale (U1036), the University Grenoble-Alpes, Commissariat à l’Energie Atomique (DSV/iRTSV/BCI), the Région Rhône-Alpes, the Ligue Nationale contre le Cancer, and the Ligue Départementale (Isère) contre le Cancer.
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Reynaud, D., Sergent, F., Abi Nahed, R., Brouillet, S., Benharouga, M., Alfaidy, N. (2018). EG-VEGF Maintenance Over Early Gestation to Develop a Pregnancy-Induced Hypertensive Animal Model. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_25
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DOI: https://doi.org/10.1007/978-1-4939-7498-6_25
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7498-6
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