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In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases

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Preeclampsia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1710))

Abstract

Lack of blood flow and aberrant levels of oxygenation in placentas are recurrent in pregnancy diseases, such as preeclampsia. These alterations generate situations of hypoxia and hypoxia/reoxygenation (H/R) and consequent oxidative stress, increased cell death, and inflammation in trophoblasts. The models used to understand the effects of hypoxia and H/R on trophoblasts require a rather big structure. This chapter describes the details of a suitable and reasonable approach with hypoxia chambers to expose human placental trophoblasts to variable conditions of oxygenation.

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Acknowledgments

This work was supported financially by the discovery grant of the Natural Sciences and Engineering Research Council of Canada (NSERC) (no. 262011-2009) and by the March of Dimes Foundation. L.S.F. received a studentship from Fonds Québécois de la Recherche sur la Nature et les Technologies (FQRNT).

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Authors and Affiliations

  1. INRS – Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC, Canada, H7V 1B7

    Lucas Sagrillo-Fagundes, Laetitia Laurent, Josianne Bienvenue-Pariseault & Cathy Vaillancourt

  2. BioMed Research Centre, 531 Boulevard des Prairies, Laval, QC, Canada, H7V 1B7

    Lucas Sagrillo-Fagundes, Laetitia Laurent, Josianne Bienvenue-Pariseault & Cathy Vaillancourt

  3. Center for Interdisciplinary Research on Well-Being, Health, Society and Environment, Université du Québec à Montréal, Montréal, QC, Canada

    Lucas Sagrillo-Fagundes, Laetitia Laurent, Josianne Bienvenue-Pariseault & Cathy Vaillancourt

Authors
  1. Lucas Sagrillo-Fagundes
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  2. Laetitia Laurent
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  3. Josianne Bienvenue-Pariseault
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  4. Cathy Vaillancourt
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Corresponding author

Correspondence to Cathy Vaillancourt .

Editor information

Editors and Affiliations

  1. Monash Medical Centre, Monash University, Clayton, Victoria, Australia

    Padma Murthi

  2. INRS-Institut Armand-Frappier, Laval, Québec, Canada

    Cathy Vaillancourt

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Sagrillo-Fagundes, L., Laurent, L., Bienvenue-Pariseault, J., Vaillancourt, C. (2018). In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7498-6_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7497-9

  • Online ISBN: 978-1-4939-7498-6

  • eBook Packages: Springer Protocols

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