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Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides

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Chaperones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1709))

Abstract

Human HspB1 (also denoted as Hsp27) belongs to the family of small (or stress) proteins (sHsps). The family, which contains ten members including αA,B-crystallin polypeptides, is characterized by a conserved C-terminal α-crystallin domain and molecular weights ranging from 20 to 40 kDa. Here, procedures are described for analyzing the dynamic oligomerization and phosphorylation patterns of HspB1 in cells exposed to different environments. Changes in the structural organization of HspB1 can reprogram its interaction with specific partner/client polypeptides. Methods are presented to analyze these interactions using tissue culture cells genetically modified to express different levels of this protein. In addition, the laboratory approaches presented here could be used to test the nine other human sHsp members as well as sHsps from other species.

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Abbreviations

Hsp27/B1:

Heat shock protein 27/B1

Hsps:

heat shock proteins

sHsp:

small stress proteins

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Authors and Affiliations

  1. Cancer and Development Laboratory, Lyon Cancer Research Center, Centre Léon Bérard, UMR INSERM 1052-CNRS 5286, Claude Bernard University, 28 rue Laennec, Lyon, 69008, France

    André-Patrick Arrigo

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  1. André-Patrick Arrigo
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Correspondence to André-Patrick Arrigo .

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Editors and Affiliations

  1. Department of Radiation, Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA

    Stuart K. Calderwood

  2. Department of Urology, Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania, USA

    Thomas L. Prince

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Arrigo, AP. (2018). Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides. In: Calderwood, S., Prince, T. (eds) Chaperones. Methods in Molecular Biology, vol 1709. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7477-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7477-1_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7476-4

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