Abstract
Transaminases are efficient tools for the stereoselective conversion of prochiral ketones into valuable chiral amines. Notably, the diversity of naturally occurring α-transaminases offers access to a wide range of l- and d-α-amino acids. We describe here two continuous colorimetric assays for the quantification of transamination activities between a keto acid and a standard donor substrate (l- or d-Glutamic acid or cysteine sulfinic acid). These assays are helpful for kinetic studies as well as for high-throughput screening of enzyme collections.
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Heuson, E., Petit, JL., Charmantray, F., de Bérardinis, V., Gefflaut, T. (2018). Continuous High-Throughput Colorimetric Assays for α-Transaminases. In: Bornscheuer, U., Höhne, M. (eds) Protein Engineering. Methods in Molecular Biology, vol 1685. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7366-8_13
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DOI: https://doi.org/10.1007/978-1-4939-7366-8_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7364-4
Online ISBN: 978-1-4939-7366-8
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