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Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection

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Wheat Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1679))

Abstract

Endogenous reference genes (ERG) and their derivate analytical methods are standard requirements for analysis of genetically modified organisms (GMOs). Development and validation of suitable ERGs is the primary step for establishing assays that monitoring the genetically modified (GM) contents in food/feed samples. Herein, we give a review of the ERGs currently used for GM wheat analysis, such as ACC1, PKABA1, ALMT1, and Waxy-D1, as well as their performances in GM wheat analysis. Also, we discussed one model for developing and validating one ideal RG for one plant species based on our previous research work.

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Author information

Authors and Affiliations

  1. National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-Bor Luh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China

    Litao Yang, Sheng Quan & Dabing Zhang

Authors
  1. Litao Yang
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  2. Sheng Quan
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  3. Dabing Zhang
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Corresponding author

Correspondence to Dabing Zhang .

Editor information

Editors and Affiliations

  1. Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia

    Prem L. Bhalla

  2. Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia

    Mohan B. Singh

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Yang, L., Quan, S., Zhang, D. (2017). Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection. In: Bhalla, P., Singh, M. (eds) Wheat Biotechnology. Methods in Molecular Biology, vol 1679. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7337-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7337-8_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7335-4

  • Online ISBN: 978-1-4939-7337-8

  • eBook Packages: Springer Protocols

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