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Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics

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Super-Resolution Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1663))

Abstract

Fluorescence microscopy is an essential tool for imaging tagged biological structures. Due to the wave nature of light, the resolution of a conventional fluorescence microscope is limited laterally to about 200 nm and axially to about 600 nm, which is often referred to as the Abbe limit. This hampers the observation of important biological structures and dynamics in the nano-scaled range ~10 nm to ~100 nm. Consequentially, various methods have been developed circumventing this limit of resolution. Super-resolution microscopy comprises several of those methods employing physical and/or chemical properties, such as optical/instrumental modifications and specific labeling of samples. In this article, we will give a brief insight into a variety of selected optical microscopy methods reaching super-resolution beyond the Abbe limit. We will survey three different concepts in connection to biological applications in radiation research without making a claim to be complete.

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Authors and Affiliations

  1. High-Content Analysis of the Cell (HiCell) and Advanced Biological Screening Facility, BioQuant, Heidelberg University, Heidelberg, Germany

    Jan Philipp Eberle, Manuel Gunkel & Holger Erfle

  2. Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany

    Alexander Rapp

  3. Kirchhoff-Institute for Physics, Heidelberg University, In the Neuenheimer Feld 227, 69120, Heidelberg, Germany

    Matthias Krufczik, Marion Eryilmaz & Michael Hausmann

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  1. Jan Philipp Eberle
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  2. Alexander Rapp
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  4. Marion Eryilmaz
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  5. Manuel Gunkel
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  6. Holger Erfle
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Correspondence to Michael Hausmann .

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  1. High-Content Analysis of the Cell (HiCell) and Advanced Biological Screening Facility, BioQuant, Heidelberg University, Heidelberg, Germany

    Holger Erfle

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Eberle, J.P. et al. (2017). Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics. In: Erfle, H. (eds) Super-Resolution Microscopy. Methods in Molecular Biology, vol 1663. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7265-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7265-4_1

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