Abstract
miRNAs are small noncoding RNA molecules that function as regulators of gene expression. Deregulated miRNA expression has been reported in various diseases including cancer. Due to their small size and high degree of homology, accurate quantification of miRNA expression is technically challenging. In this chapter, we present two different technologies for miRNA quantification: small RNA sequencing and RT-qPCR.
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Van Goethem, A., Mestdagh, P., Van Maerken, T., Vandesompele, J. (2017). MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation. In: Kaufmann, M., Klinger, C., Savelsbergh, A. (eds) Functional Genomics. Methods in Molecular Biology, vol 1654. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7231-9_13
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DOI: https://doi.org/10.1007/978-1-4939-7231-9_13
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