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Estimation of Photorespiratory Fluxes by Gas Exchange

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Photorespiration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1653))

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Abstract

Photorespiratory fluxes can be easily estimated by photosynthetic gas exchange using an infrared gas analyzer and applying the Farquhar, von Caemmerer, and Berry (Farquhar et al. Planta 149:78–90, 1980) photosynthesis model. For a more direct measurement of photorespiratory CO2 release from glycine decarboxylation, infrared gas analysis can be coupled to membrane-inlet mass spectrometry, capable of separating the total CO2 concentration into its 12CO2 and 13CO2 components in a continuous online fashion. This chapter discusses how to calculate rates of photorespiration from Rubisco kinetics and describes in detail a method for measuring the CO2 release from glycine decarboxylation using 13CO2.

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References

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Acknowledgements

F.A.B. is supported by funding from the ARC Centre of Excellence for Translational Photosynthesis awarded to Graham Farquhar.

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Authors and Affiliations

  1. Research School of Biology, The Australian National University, Acton, Canberra, ACT, 2601, Australia

    Florian A. Busch, Ross M. Deans & Meisha-Marika Holloway-Phillips

Authors
  1. Florian A. Busch
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  2. Ross M. Deans
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  3. Meisha-Marika Holloway-Phillips
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Corresponding author

Correspondence to Florian A. Busch .

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Editors and Affiliations

  1. Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

    Alisdair R. Fernie

  2. Plant Physiology Department, University of Rostock, Rostock, Germany

    Hermann Bauwe

  3. Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Düsseldorf, Germany

    Andreas P.M. Weber

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Busch, F.A., Deans, R.M., Holloway-Phillips, MM. (2017). Estimation of Photorespiratory Fluxes by Gas Exchange. In: Fernie, A., Bauwe, H., Weber, A. (eds) Photorespiration. Methods in Molecular Biology, vol 1653. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7225-8_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7225-8_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7224-1

  • Online ISBN: 978-1-4939-7225-8

  • eBook Packages: Springer Protocols

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