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  • © 2017

Photorespiration

Methods and Protocols

  • Includes cutting-edge and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1653)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
  2. Estimation of Photorespiratory Fluxes by Gas Exchange

    • Florian A. Busch, Ross M. Deans, Meisha-Marika Holloway-Phillips
    Pages 1-15
  3. Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling

    • Amna Mhamdi, Pavel I. Kerchev, Patrick Willems, Graham Noctor, Frank Van Breusegem
    Pages 17-29
  4. Measurement of Enzyme Activities

    • Hermann Bauwe
    Pages 31-50
  5. In Vitro Alkylation Methods for Assessing the Protein Redox State

    • Flavien Zannini, Jérémy Couturier, Olivier Keech, Nicolas Rouhier
    Pages 51-64
  6. Dimethyl-Labeling-Based Quantification of the Lysine Acetylome and Proteome of Plants

    • Ines Lassowskat, Markus Hartl, Fabian Hosp, Paul J. Boersema, Matthias Mann, Iris Finkemeier
    Pages 65-81
  7. In Vitro Analysis of Metabolite Transport Proteins

    • Marc-Sven Roell, Franziska Kuhnert, Shirin Zamani-Nour, Andreas P. M. Weber
    Pages 83-96
  8. Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration

    • Stefan Timm, Katharina Modde, Hermann Bauwe
    Pages 105-124
  9. Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits

    • Franklin Magnum de Oliveira Silva, Lucas de Ávila Silva, Wagner L. Araújo, Agustin Zsögön, Adriano Nunes-Nesi
    Pages 125-135
  10. Metabolic Engineering of Photorespiration

    • Martin K. M. Engqvist, Veronica G. Maurino
    Pages 137-155
  11. 13CO2 Labeling and Mass Spectral Analysis of Photorespiration

    • Leonardo Perez de Souza, Marek Szecówka, Alisdair R. Fernie, Takayuki Tohge
    Pages 157-166
  12. Isotopically Nonstationary Metabolic Flux Analysis (INST-MFA) of Photosynthesis and Photorespiration in Plants

    • Fangfang Ma, Lara J. Jazmin, Jamey D. Young, Doug K. Allen
    Pages 167-194
  13. Genome-Scale Modeling of Photorespiratory Pathway Manipulation

    • Anika Küken, Zoran Nikoloski
    Pages 195-202
  14. Kinetic Modeling of Photorespiration

    • Honglong Zhao, Yi Xiao, Xin-Guang Zhu
    Pages 203-216
  15. Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues

    • Alisdair R. Fernie, Hermann Bauwe, Lee J. Sweetlove
    Pages 217-225
  16. Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana

    • Stephan Krueger, Ruben M. Benstein, Sabine Wulfert, Armand D. Anoman, María Flores-Tornero, Roc Ros
    Pages 227-242
  17. Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration

    • Roxana Khoshravesh, Vanessa Lundsgaard-Nielsen, Stefanie Sultmanis, Tammy L. Sage
    Pages 243-270
  18. Back Matter

    Pages 271-274

About this book

This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

    Alisdair R. Fernie

  • Plant Physiology Department, University of Rostock, Rostock, Germany

    Hermann Bauwe

  • Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University, Düsseldorf, Germany

    Andreas P.M. Weber

Bibliographic Information

  • Book Title: Photorespiration

  • Book Subtitle: Methods and Protocols

  • Editors: Alisdair R. Fernie, Hermann Bauwe, Andreas P.M. Weber

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7225-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7224-1Published: 19 August 2017

  • Softcover ISBN: 978-1-4939-8422-0Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7225-8Published: 17 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 274

  • Number of Illustrations: 15 b/w illustrations, 25 illustrations in colour

  • Topics: Plant Sciences

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Pharma

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access