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Gender Identification in Date Palm Using Molecular Markers

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Date Palm Biotechnology Protocols Volume II

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1638))

Abstract

Breeding of date palm is complicated because of its long life cycle and heterozygous nature. Sexual propagation of date palm does not produce true-to-type plants. Sex of date palms cannot be identified until the first flowering stage. Molecular markers such as random amplified polymorphic DNA (RAPD), sequence-characterized amplified regions (SCAR), and simple sequence repeats (SSR) have successfully been used to identify the sex-linked loci in the plant genome and to isolate the corresponding genes. This chapter highlights the use of three molecular markers including RAPD, SCAR, and SSR to identify the gender of date palm seedlings.

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Acknowledgment

This research work was funded by the Higher Education Commission, Pakistan, and supported by Plant Functional Genomics Lab., Centre of Agricultural Biochemistry and Biotechnology (CABB), and Plant Tissue Culture Cell, University of Agriculture Faisalabad, Pakistan.

Author information

Authors and Affiliations

  1. Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, 3804, Pakistan

    Faisal Saeed Awan

  2. Institute of Horticultural Sciences, University of Agriculture, Faisalabad, 38040, Pakistan

    Maryam & Muhammad J. Jaskani

  3. U.S.-Pakistan Center for Advanced Studies in Agriculture and Food Security (USPCAS-AFS), University of Agriculture, Faisalabad, Pakistan

    Bushra Sadia

Authors
  1. Faisal Saeed Awan
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  2. Maryam
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  3. Muhammad J. Jaskani
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  4. Bushra Sadia
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Corresponding author

Correspondence to Faisal Saeed Awan .

Editor information

Editors and Affiliations

  1. Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, Saudi Arabia

    Jameel M. Al-Khayri

  2. Agricultural Sciences, University of Helsinki, Helsinki, Finland

    S.Mohan Jain

  3. Cincinnati, Ohio, USA

    Dennis V. Johnson

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Awan, F.S., Maryam, Jaskani, M.J., Sadia, B. (2017). Gender Identification in Date Palm Using Molecular Markers. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_18

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  • DOI: https://doi.org/10.1007/978-1-4939-7159-6_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7158-9

  • Online ISBN: 978-1-4939-7159-6

  • eBook Packages: Springer Protocols

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