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Date Palm Genetic Diversity Analysis Using Microsatellite Polymorphism

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Date Palm Biotechnology Protocols Volume II

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1638))

Abstract

Date palm (Phoenix dactylifera L.) is considered one of the great socioeconomic resources in the Middle East and the Arab regions. The tree has been and still is at the center of the comprehensive agricultural development. The number of known date palm cultivars, distributed worldwide, is approximately 3000. The success of genetic diversity conservation or any breeding program depends on an understanding of the amount and distribution of the genetic variation already in existence in the genetic pool. Development of suitable DNA molecular markers for this tree may allow researchers to estimate genetic diversity, which will ultimately lead to the genetic conservation of date palm. Simple sequence repeats (SSRs) are DNA strands, consisting of tandemly repeated mono-, di-, tri-, tetra-, or penta-nucleotide units that are arranged throughout the genomes of most eukaryotic species. Microsatellite markers, developed from genomic libraries, belong to either the transcribed region or the non-transcribed region of the genome, and there is rarely available information on their functions. Microsatellite sequences are especially suited to distinguish closely related genotypes due to a high degree of variability making them ideally suitable in population studies and the identification of closely related cultivars. This chapter focuses on the methods employed to characterize date palm genotypes using SSR markers.

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Acknowledgment

This project was supported by a grant from Tottori University and funds from Bundesministerium für Wirtschaftliche Zusammenarbeit und Entwicklung (BMZ), Germany, and the State Board of the Agriculture Research, Ministry of Agriculture, Iraq. We acknowledge the technical support of A. Sabbagh of the International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria.

Author information

Authors and Affiliations

  1. Date Palm Research Unit, College of Agriculture, University of Baghdad, P.O. Box 47054, Baghdad, Iraq

    Hussam S. M. Khierallah

  2. State Board of Agricultural Research, Ministry of Agriculture, Baghdad, Iraq

    Saleh M. Bader

  3. International Center for Agricultural Research in the Dry Areas (ICARDA), Cairo, Egypt

    Alladin Hamwieh & Michael Baum

Authors
  1. Hussam S. M. Khierallah
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  2. Saleh M. Bader
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  3. Alladin Hamwieh
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  4. Michael Baum
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Corresponding author

Correspondence to Hussam S. M. Khierallah .

Editor information

Editors and Affiliations

  1. Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, Saudi Arabia

    Jameel M. Al-Khayri

  2. Agricultural Sciences, University of Helsinki, Helsinki, Finland

    S.Mohan Jain

  3. Cincinnati, Ohio, USA

    Dennis V. Johnson

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Khierallah, H.S.M., Bader, S.M., Hamwieh, A., Baum, M. (2017). Date Palm Genetic Diversity Analysis Using Microsatellite Polymorphism. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7159-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7158-9

  • Online ISBN: 978-1-4939-7159-6

  • eBook Packages: Springer Protocols

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