Abstract
Pollen storage and viability are very important for pollination, breeding, biodiversity, biotechnology, conservation, and other biological and non-biological studies of the date palm. Optimizing procedures and duration of storage are important for effective and long-term date palm pollen storage and viability. Here we describe pollen storage methods, such as room temperature (25–30 °C), refrigeration (4 °C), storage at 4 °C in desiccators, deep freezer (−20 °C), and cryopreservation (−196 °C). Based on pollen viability by staining and in vitro germination methods, cryopreservation is the best method for long-term storage without any significant effect on pollen viability (75–84%); however, the percentage of pollen viability depends on the storage period.
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References
Shivanna KR, Linkens HF, Cresti M (1991) Pollen viability and pollen vigor. Theor Appl Genet 81:38–42
Ibrahim AMF, Kobbia EL (1998) Influence of the storage periods on pollen viability of four date male types. Alex J Agric Res 43:247–257
Gupta MR, Thatai SK (1980) Storage of date pollen. Punjab Hort J 20:211–214
Boughdeiri L, Boungaga N (1991) Storage of date palm pollen (Phoenix dactylifera L.). 1. preliminary results. Ann Sci Nat 11:119–124
Maryam JMJ, Fatima B, Haider MS, Naqvi SA, Nafees M et al (2015) Evaluation of pollen viability in date palm cultivars under different storage temperatures. Pak J Bot 47:377–381
Shaheen MA (1983) Identification of some seedling male date palms by pollen ultrastructure. J Coll Agric King Saud Univ 5:137–142
Ateyyeh AF (2012) Effect of storage method on date palm and pistachio pollen viability. Jordan J Agric Sci 8:573–582
Silva ED, Rodrigues LR, Mariath JEA (2011) Contradictory results in pollen viability determination of Valeriana scandens L. Geneconserve 40:234–242
Shaheen MA (2004) Evaluation of date palm males using pollen viability and ultrastructure. Acta Hortic 632:37–43
Al-Helal AA, Basalah MO, Muhammad S (1988) Effect of storage and temperature on pollen germination and range of pollen tube elongation of date palm (Phoenix dactylifera L.) Phyton Buenos Aires 48:119–122
Tisserat B, Ulrich JM, Finkle BJ (1981) Survival of Phoenix pollen grains under cryogenic conditions. J Crop Sci 23:461–470
Bolat J, Pirlak L (1999) An investigation on pollen viability, germination and tube growth in some stone fruits. J Agric Forest 23:383–389
Rodriguez-Riano T, Dafni A (2000) A new procedure to assess pollen viability. Sex Plant Reprod 12:241–244
Boughediri L, Bounaga N (1987) In vitro germination of date pollen and its relation to fruit set. Date Palm J 5:120–127
Acknowledgment
This work was supported by the Higher Education Commission, Pakistan and Plant Tissue Culture Cell, University of Agriculture, Faisalabad 38040, Pakistan.
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Maryam, Jaskani, M.J., Naqvi, S.A. (2017). Storage and Viability Assessment of Date Palm Pollen. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_1
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DOI: https://doi.org/10.1007/978-1-4939-7159-6_1
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7159-6
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