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Enhanced Indirect Somatic Embryogenesis of Date Palm Using Low Levels of Seawater

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Date Palm Biotechnology Protocols Volume I

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1637))

Abstract

Date palm tolerates salinity, drought, and high temperatures. Arid and semiarid zones, especially the Middle East region, need a huge number of date palms for cultivation. To meet this demand, tissue culture techniques have great potential for mass production of plantlets, especially using the indirect embryogenesis technique; any improvement of these techniques is a worthy objective. Low levels of salinity can enhance growth and development of tolerant plants. A low level of seawater, a natural source of salinity, reduces the time required for micropropagation processes of date palm cv. Malkaby when added to MS medium. Medium containing seawater at 500 ppm total dissolved solid (TDS) (12.2 mL/L) improves callus proliferation, whereas 1500 ppm (36.59 mL/L) enhances plant regeneration including multiplication of secondary embryos, embryo germination, and rooting.

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Correspondence to Rania A. Taha .

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Taha, R.A. (2017). Enhanced Indirect Somatic Embryogenesis of Date Palm Using Low Levels of Seawater. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7156-5_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7155-8

  • Online ISBN: 978-1-4939-7156-5

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