Abstract
Hyperhydricity (or vitrification) is a fundamental physiological disorder in date palm micropropagation. Several factors have been ascribed as being responsible for hyperhydricity, which are related to the explant, medium, culture vessel, and environment. The optimization of inorganic nutrients in the culture medium improves in vitro growth and morphogenesis, in addition to controlling hyperhydricity. This chapter describes a protocol for controlling hyperhydricity during the embryogenic callus stage by optimizing the ratio of nitrogen salts of the Murashige and Skoog (MS) nutrient culture medium. The best results of differentiation from cured hyperhydric callus are obtained using modification at a ratio of NH4+/NO3− at 10:15 (825:1425 mg/L) of the MS culture medium to remedy hyperhydric date palm callus and achieve the recovery of normal embryogenic callus and subsequent regeneration of plantlets. Based on the results of this study, nutrient medium composition has an important role in avoiding hyperhydricity problems during date palm micropropagation.
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References
McCubbin MJ, Zaid A (2007) Would a combination of organogenesis and embryogenesis techniques in date palm micropropagation be the answer? Acta Hortic 736:255–259
Mazri MA, Meziani R (2013) An improved method for micropropagation and regeneration of date palm Phoenix dactylifera L. J Plant Biochem Biotechnol 22:176–184
Mazri MA (2015) Role of cytokinins and physical state of the culture medium to improve in vitro shoot multiplication, rooting and acclimatization of date palm Phoenix dactylifera L. cv. Boufeggous. J Plant Biochem Biotechnol 24:268–275
Al-Khateeb AA (2008) The problems facing the use of tissue culture technique in date palm Phoenix dactylifera L. Sci J King Faisal Univ 9:85–104
Oliveira Y, Pinto F, Silva AL, Guedes I, Biasi LA, Quoirin M (2010) An efficient protocol for micropropagation of Melaleuca alternifolia Cheel. In Vitro Cell Dev Biol Plant 46:192–197
Reed BM, Wada S, De Noma J, Niedz RP (2013) Mineral nutrition influences physiological responses of pear in vitro. In Vitro Cell Dev Biol Plant 49:699–709
Cassells A, Curry R (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tiss Org Cult 64(2–3):145–157
Tian J, Cheng Y, Kong X, Liu M, Jiang F, Wu Z (2016) Induction of reactive oxygen species and the potential role of NADPH oxidase in hyperhydricity of garlic plantlets in vitro. Protoplasma 5:1–10
Zayed ZE, El-Dawayaty MM, Abdel-Gelil LM (2012) Hyperhydricity-phenomenon problem in embryogenic callus of date palm, solving by glutamine and NH4+:NO3− ratio in basal nutrient medium. Egypt J Biotechnol 42:86–95
Zayed ZE, Sidky RA, Saber TY (2013) Prevention of hyperhydricity phenomenon and improving somatic embryogenesis in date palm Phoenix dactylifera L. Bull Fac Agric Cairo Univ 64:297–302
Sidky RA, Gadalla EG (2014) Somatic embryogenesis in Phoenix dactylifera maturation, germination and reduction of hyperhydricity during embryogenic cell suspension culture. In: Zaid A, Alhadrami GA (eds) Proceedings of the fifth international date palm conference, Khalifa International Date Palm Award, Abu Dhabi, UAE, pp 183–190
El - Dawayati MM, Abd El Bar OH, Zayed ZE, El Din AFM Z (2012) In vitro morpho-histological studies of new developed embryos from abnormal malformed embryos of date palm cv. Gundila under desiccation effect of polyethelyne glycol treatments. Ann Agr Sci 57(2):95–106
El - Dawayati MM, Zayed ZE, Ibrahim IA (2013) In vitro morphogenesis changes and embryogenesis of recovered abnormal embryos of date palm under effect of air desiccation treatments. Arab J Biotech 16(1):105–118
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
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El-Dawayati, M.M., Zayed, Z.E. (2017). Controlling Hyperhydricity in Date Palm In Vitro Culture by Reduced Concentration of Nitrate Nutrients. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_15
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DOI: https://doi.org/10.1007/978-1-4939-7156-5_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7156-5
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