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Deconstructing the Metabolic Networks of Oncogenic Signaling Using Targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

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Kinase Signaling Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1636))

Abstract

Metabolic reprogramming is recognized as an emerging hallmark of oncogenic signaling and cancer development. Hence the need to identify novel quantitative analytical platforms for studying metabolism in vitro and in vivo has dramatically increased. Here, we describe the experimental workflow for a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach involving positive/negative ion switching to analyze >250 metabolites of central carbon metabolism, nucleotides, and amino acids.

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Author information

Authors and Affiliations

  1. Division of Cancer Biology, The Institute of Cancer Research, London, UK

    George Poulogiannis

Authors
  1. George Poulogiannis
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Correspondence to George Poulogiannis .

Editor information

Editors and Affiliations

  1. Division of Medical Oncology, University of Colorado, Aurora, Colorado, USA

    Aik-Choon Tan

  2. Protein Networks Laboratory, Institute of Cancer Research, London, United Kingdom

    Paul H. Huang

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Poulogiannis, G. (2017). Deconstructing the Metabolic Networks of Oncogenic Signaling Using Targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). In: Tan, AC., Huang, P. (eds) Kinase Signaling Networks. Methods in Molecular Biology, vol 1636. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7154-1_26

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  • DOI: https://doi.org/10.1007/978-1-4939-7154-1_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7152-7

  • Online ISBN: 978-1-4939-7154-1

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