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Labeling of Membrane Complexes for Electron Microscopy

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Membrane Protein Structure and Function Characterization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1635))

Abstract

Localization of specific subunits or domains of interest inside protein complexes can be challenging, especially for membrane machineries. The amphipatic nature of their subunits and their modular organization results in difficult genetic manipulation and instability upon purification. Here, we present different labeling approaches that have been demonstrated successful in the structural characterization of large membrane complexes.

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Acknowledgments

The authors acknowledge Dr. Harry Low and Pr. Gabriel Waksman for their contribution on the T4SS section. This work was supported by funds from the Institut Pasteur, the CNRS, and the ERC.

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Authors and Affiliations

  1. Unite´ G5 Biologie structurale de la sécrétion bactérienne, Institut Pasteur, 25–28 rue du Docteur Roux, 75015, Paris, France

    Francesca Gubellini & Rémi Fronzes

  2. UMR 3528, CNRS, Institut Pasteur, 25–28 rue du Docteur Roux, 75015, Paris, France

    Francesca Gubellini & Rémi Fronzes

  3. European Institute of Chemistry and Biology, University of Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France

    Rémi Fronzes

  4. CNRS UMR 5234 Microbiologie Fondamentale et Pathogénicité, Paris, France

    Rémi Fronzes

  5. Unité de Microbiologie Structurale, Institut Pasteur, 25 rue du Docteur Roux, 75015, Paris, France

    Francesca Gubellini

Authors
  1. Francesca Gubellini
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  2. Rémi Fronzes
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Corresponding author

Correspondence to Francesca Gubellini .

Editor information

Editors and Affiliations

  1. Département de Chimie, CNRS UMR 7203 LBM, Sorbonne Universités—UPMC Univ Paris 06, École Normale Supérieure—PSL Research University, Paris, France

    Jean-Jacques Lacapere

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Gubellini, F., Fronzes, R. (2017). Labeling of Membrane Complexes for Electron Microscopy. In: Lacapere, JJ. (eds) Membrane Protein Structure and Function Characterization. Methods in Molecular Biology, vol 1635. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7151-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7151-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7149-7

  • Online ISBN: 978-1-4939-7151-0

  • eBook Packages: Springer Protocols

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