Membrane Protein Structure and Function Characterization

Methods and Protocols

  • Jean-Jacques Lacapere

Part of the Methods in Molecular Biology book series (MIMB, volume 1635)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Lucile Senicourt, Soria Iatmanen-Harbi, Claude Hattab, Mariano Anibal Ostuni, Marie-France Giraud, Jean-Jacques Lacapere
    Pages 1-25
  3. Hajer Souabni, Aymen Ezzine, Tania Bizouarn, Laura Baciou
    Pages 27-43
  4. Lucie Hartmann, Estelle Metzger, Noémie Ottelard, Renaud Wagner
    Pages 45-56
  5. Isabelle Larrieu, James Tolchard, Corinne Sanchez, Edmond Yazo Kone, Alexandre Barras, Claire Stines-Chaumeil et al.
    Pages 57-90
  6. Marie-Laure Fogeron, Aurélie Badillo, François Penin, Anja Böckmann
    Pages 91-108
  7. Vilius Kurauskas, Paul Schanda, Remy Sounier
    Pages 109-123
  8. Francesca Gubellini, Rémi Fronzes
    Pages 125-138
  9. Ghérici Hassaïne, Cédric Deluz, Luigino Grasso, Romain Wyss, Ruud Hovius, Henning Stahlberg et al.
    Pages 139-168
  10. Batoul Srour, Stefan Bruechert, Susana L. A. Andrade, Petra Hellwig
    Pages 195-203
  11. Jeroen F. van Dyck, Albert Konijnenberg, Frank Sobott
    Pages 205-232
  12. Luc Negroni, Michel Zivy, Claire Lemaire
    Pages 233-246
  13. Alice Verchère, Isabelle Broutin, Martin Picard
    Pages 259-282
  14. Michel Vivaudou, Zlatomir Todorov, Gina Catalina Reyes-Mejia, Christophe Moreau
    Pages 283-301
  15. Philippe Rosier, Frédéric Gélébart, Nicolas Dumesnil, Gauthier Esnot, Manuela Dezi, Marc Morand et al.
    Pages 303-316
  16. Laetitia Daury, Jean-Christophe Taveau, Dimitri Salvador, Marie Glavier, Olivier Lambert
    Pages 317-327
  17. Lucile Senicourt, Luminita Duma, Vassilios Papadopoulos, Jean-Jacques Lacapere
    Pages 329-344
  18. Denis Lacabanne, Britta Kunert, Carole Gardiennet, Beat H. Meier, Anja Bo¨ckmann
    Pages 345-358

About this book

Introduction

In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins.  Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field. 

Keywords

membrane protein folded protein membrane mimicking environments functional status antibodies

Editors and affiliations

  • Jean-Jacques Lacapere
    • 1
  1. 1.Département de Chimie, CNRS UMR 7203 LBMSorbonne Universités—UPMC Univ Paris 06, École Normale Supérieure—PSL Research UniversityParisFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7151-0
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7149-7
  • Online ISBN 978-1-4939-7151-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Chemical Manufacturing
Biotechnology
Consumer Packaged Goods
Pharma