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Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins

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Membrane Protein Structure and Function Characterization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1635))

Abstract

Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in E. coli-based protein expression, as exemplified with the mitochondrial carrier GGC.

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Acknowledgments

This work was financially supported by the European Research Council (ERC-Stg-2012- 311318-ProtDyn2Function). We would like to thank Pr. J. J. Chou and Dr. K. Oxenoid for stimulating discussions, and also Dr. J. Boisbouvier and Dr. R. Kerfah for helpful insights on labeling protocols.

Author information

Authors and Affiliations

  1. IBS, Université Grenoble Alpes, F-38044, Grenoble, France

    Vilius Kurauskas & Paul Schanda

  2. CEA, Institut de Biologie Structurale, F-38044, Grenoble, France

    Vilius Kurauskas & Paul Schanda

  3. CNRS, Institut de Biologie Structurale, F-38044, Grenoble, France

    Vilius Kurauskas & Paul Schanda

  4. Institut de Génomique Fonctionnelle (IGF), CNRS, INSERM, Univ. Montpellier, F-34094, Montpellier, France

    Remy Sounier

Authors
  1. Vilius Kurauskas
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  2. Paul Schanda
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  3. Remy Sounier
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Corresponding author

Correspondence to Remy Sounier .

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Editors and Affiliations

  1. Département de Chimie, CNRS UMR 7203 LBM, Sorbonne Universités—UPMC Univ Paris 06, École Normale Supérieure—PSL Research University, Paris, France

    Jean-Jacques Lacapere

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Kurauskas, V., Schanda, P., Sounier, R. (2017). Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins. In: Lacapere, JJ. (eds) Membrane Protein Structure and Function Characterization. Methods in Molecular Biology, vol 1635. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7151-0_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7151-0_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7149-7

  • Online ISBN: 978-1-4939-7151-0

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