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Modeling Ebolavirus Budding with Virus Like Particles

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Ebolaviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1628))

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Abstract

About 15 years ago, several groups initially described the release of virus like particles (VLPs) upon expression of Ebola virus VP40 in mammalian cells. Further development of the protocol later allowed for the dissection of the Ebola virus budding mechanism and for the identification of critical VP40 residues involved in this process. VLPs are now produced routinely in several laboratories as a tool to study virus entry or egress and have even been proposed as vaccine candidates against Ebola virus disease. Here we described protocols for the production and the analysis of Ebola virus VLP release.

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Author information

Authors and Affiliations

  1. INSERM U1111—CNRS UMR5308, Centre International de Recherche en Infectiologie (CIRI), Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Lyon, France

    Olivier Reynard & Mathieu Mateo

  2. UBIVE, Institut Pasteur, CIRI, Lyon, France

    Mathieu Mateo

Authors
  1. Olivier Reynard
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  2. Mathieu Mateo
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Corresponding author

Correspondence to Olivier Reynard .

Editor information

Editors and Affiliations

  1. Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

    Thomas Hoenen

  2. Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany

    Allison Groseth

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Reynard, O., Mateo, M. (2017). Modeling Ebolavirus Budding with Virus Like Particles. In: Hoenen, T., Groseth, A. (eds) Ebolaviruses. Methods in Molecular Biology, vol 1628. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7116-9_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7116-9_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7115-2

  • Online ISBN: 978-1-4939-7116-9

  • eBook Packages: Springer Protocols

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