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Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization

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Diagnostic Bacteriology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1616))

Abstract

In this chapter, we describe a fluorescence in vivo hybridization (FIVH) protocol, using nucleic acid probes, for the detection of the bacterium Helicobacter pylori in the gastric mucosa of an infected C57BL/6 mouse model. This protocol should be easily extended to other microorganisms not only as a way to identify in vivo important microorganisms and their patterns of distribution within specific or at different anatomic sites, but also to better understand interaction mechanisms involving the microbiome and the human body.

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Acknowledgments

This work was financially supported by Project UID/EQU/00511/2013-LEPABE, by the FCT/MEC with national funds and when applicable cofunded by FEDER in the scope of the P2020 Partnership Agreement; by FEDER funds through Programa Operacional Factores de Competitividade—COMPETE, by the Programa Operacional do Norte (ON2) program, Project NORTE-07-0124-FEDER-000022 to M.L., and by national funds through FCT (Fundação para a Ciência e a Tecnologia): DNA mimics Research Project PIC/IC/82815/2007, Ph.D. grant, SFRH/BD/72999/2010 to S.F., and Post-Doctoral fellowship SFRH/BPD/33420/2008 to M.L.

Author information

Authors and Affiliations

  1. LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal

    Silvia Fontenete & Nuno F. Azevedo

  2. i3s Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal

    Silvia Fontenete, Marina Leite & Ceu Figueiredo

  3. IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal

    Silvia Fontenete, Marina Leite & Ceu Figueiredo

  4. Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark

    Silvia Fontenete

  5. ICBAS, Institute of Biomedical Sciences Abel Salazar, University of Porto, Porto, Portugal

    Silvia Fontenete

  6. Epithelial Cell Biology Group, Cancer Cell Biology Program, Spanish National Cancer Research Centre, Madrid, 28029, Madrid, Spain

    Silvia Fontenete

  7. FMUP, Faculty of Medicine of the University of Porto, Porto, Portugal

    Ceu Figueiredo

  8. Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium

    Paul Cos

Authors
  1. Silvia Fontenete
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  2. Marina Leite
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  3. Ceu Figueiredo
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  4. Paul Cos
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  5. Nuno F. Azevedo
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Corresponding author

Correspondence to Nuno F. Azevedo .

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Editors and Affiliations

  1. Germantown, Maryland, USA

    Kimberly A. Bishop-Lilly

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Fontenete, S., Leite, M., Figueiredo, C., Cos, P., Azevedo, N.F. (2017). Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization. In: Bishop-Lilly, K. (eds) Diagnostic Bacteriology. Methods in Molecular Biology, vol 1616. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7037-7_8

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  • DOI: https://doi.org/10.1007/978-1-4939-7037-7_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7035-3

  • Online ISBN: 978-1-4939-7037-7

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