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Crystallization of Membrane Proteins: An Overview

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Protein Crystallography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1607))

Abstract

Membrane proteins are crucial components of cellular membranes and are responsible for a variety of physiological functions. The advent of new tools and technologies for structural biology of membrane proteins has led to a significant increase in the number of structures deposited to the Protein Data Bank during the past decade. This new knowledge has expanded our fundamental understanding of their mechanism of function and contributed to the drug-design efforts. In this chapter we discuss current approaches for membrane protein expression, solubilization, crystallization, and data collection. Additionally, we describe the protein quality-control assays that are often instrumental as a guideline for a shorter path toward the structure.

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Authors and Affiliations

  1. Department of Chemistry, Bridge Institute, University of Southern California, Los Angeles, CA, 90089, USA

    Andrii Ishchenko, Enrique E. Abola & Vadim Cherezov

Authors
  1. Andrii Ishchenko
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  2. Enrique E. Abola
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  3. Vadim Cherezov
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Corresponding author

Correspondence to Vadim Cherezov .

Editor information

Editors and Affiliations

  1. Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland, USA

    Alexander Wlodawer

  2. Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, Illinois, USA

    Zbigniew Dauter

  3. Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland

    Mariusz Jaskolski

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Ishchenko, A., Abola, E.E., Cherezov, V. (2017). Crystallization of Membrane Proteins: An Overview. In: Wlodawer, A., Dauter, Z., Jaskolski, M. (eds) Protein Crystallography. Methods in Molecular Biology, vol 1607. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7000-1_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7000-1_5

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  • Print ISBN: 978-1-4939-6998-2

  • Online ISBN: 978-1-4939-7000-1

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