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Protein Crystallography

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Alexander Wlodawer

    1. Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Zbigniew Dauter

    1. Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Mariusz Jaskolski

    1. Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1607)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Expression and Purification of Recombinant Proteins in Escherichia coli with a His6 or Dual His6-MBP Tag

    • Sreejith Raran-Kurussi, David S. Waugh
    Pages 1-15

  3. Protein Crystallization

    • Alexander McPherson
    Pages 17-50

  4. Advanced Methods of Protein Crystallization

    • Abel Moreno
    Pages 51-76

  5. The “Sticky Patch” Model of Crystallization and Modification of Proteins for Enhanced Crystallizability

    • Zygmunt S. Derewenda, Adam Godzik
    Pages 77-115

  6. Crystallization of Membrane Proteins: An Overview

    • Andrii Ishchenko, Enrique E. Abola, Vadim Cherezov
    Pages 117-141

  7. Locating and Visualizing Crystals for X-Ray Diffraction Experiments

    • Michael Becker, David J. Kissick, Craig M. Ogata
    Pages 143-164

  8. Collection of X-Ray Diffraction Data from Macromolecular Crystals

    • Zbigniew Dauter
    Pages 165-184

  9. Identifying and Overcoming Crystal Pathologies: Disorder and Twinning

    • Michael C. Thompson
    Pages 185-217

  10. Applications of X-Ray Micro-Beam for Data Collection

    • Ruslan Sanishvili, Robert F. Fischetti
    Pages 219-238

  11. Serial Synchrotron X-Ray Crystallography (SSX)

    • Kay Diederichs, Meitian Wang
    Pages 239-272

  12. Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources

    • Marius Schmidt
    Pages 273-294

  13. Structure Determination Using X-Ray Free-Electron Laser Pulses

    • Henry N. Chapman
    Pages 295-324

  14. Processing of XFEL Data

    • Thomas A. White
    Pages 325-347

  15. Many Ways to Derivatize Macromolecules and Their Crystals for Phasing

    • Miroslawa Dauter, Zbigniew Dauter
    Pages 349-356

  16. Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX

    • Andrea Thorn
    Pages 357-376

  17. Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis

    • Qun Liu, Wayne A. Hendrickson
    Pages 377-399

  18. Long-Wavelength X-Ray Diffraction and Its Applications in Macromolecular Crystallography

    • Manfred S. Weiss
    Pages 401-420

  19. Acknowledging Errors: Advanced Molecular Replacement with Phaser

    • Airlie J. McCoy
    Pages 421-453

  20. Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems

    • Frank DiMaio
    Pages 455-466
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Keywords

About this book

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, USA

    Alexander Wlodawer

  • Synchrotron Radiation Research Section, MCL, National Cancer Institute, Argonne National Laboratory, Argonne, USA

    Zbigniew Dauter

  • Department of Crystallography, Faculty of Chemistry, A. Mickiewicz University, Poznan, Poland

    Mariusz Jaskolski

About the editors



Bibliographic Information

  • Book Title: Protein Crystallography

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7000-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6998-2Published: 02 June 2017

  • Softcover ISBN: 978-1-4939-8363-6Published: 09 June 2018

  • eBook ISBN: 978-1-4939-7000-1Published: 01 June 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 672

  • Number of Illustrations: 28 b/w illustrations, 145 illustrations in colour

  • Topics: Protein Science

  • Industry Sectors: Biotechnology, Pharma

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