Abstract
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then developed to show the protein of interest. Here, we describe the transfer and detection of Outer surface protein A (OspA), a protein only found on the surface of Borrelia burgdorferi, the bacteria responsible for Lyme disease.
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Kim, B. (2017). Western Blot Techniques. In: Espina, V. (eds) Molecular Profiling. Methods in Molecular Biology, vol 1606. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6990-6_9
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DOI: https://doi.org/10.1007/978-1-4939-6990-6_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6989-0
Online ISBN: 978-1-4939-6990-6
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