Abstract
Cell line development aims to generate and select clones with desirable characteristics. One of the most important parameters for biopharmaceutical cell selection is cell-specific productivity (Qp) or the quantity of product produced per cell per day. Fluorescence-activated cell sorting (FACS) is a powerful, high-throughput technique that facilitates multiparametric characterization and isolation of individual cell clones from heterogeneous populations. Here, we describe a FACS-based method for section of high-producing CHO cell clones.
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Abbreviations
- FSC:
-
Forward scatter
- SSC:
-
Side scatter
- FSC-W:
-
Forward scatter-width
- FSC-H:
-
Forward scatter-height
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Gallagher, C., Kelly, P.S. (2017). Selection of High-Producing Clones Using FACS for CHO Cell Line Development. In: Meleady, P. (eds) Heterologous Protein Production in CHO Cells. Methods in Molecular Biology, vol 1603. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6972-2_9
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DOI: https://doi.org/10.1007/978-1-4939-6972-2_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6971-5
Online ISBN: 978-1-4939-6972-2
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