Skip to main content

Laser Interferometry Method as a Novel Tool in Endotoxins Research

  • Protocol
  • First Online:
Microbial Toxins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1600))

  • 1161 Accesses

Abstract

Optical properties of chemical substances are widely used at present for assays thereof in a variety of scientific disciplines. One of the measurement techniques applied in physical sciences, with a potential for novel applications in biology, is laser interferometry. This method enables to record the diffusion properties of chemical substances. Here we describe the novel application of laser interferometry in chitosan interactions with lipopolysaccharide by detection of colistin diffusion. The proposed model could be used in simple measurements of polymer interactions with endotoxins and/or biological active compounds, like antibiotics.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Arabski M, Wąsik S, Zych M et al (2013) Analysis of ciprofloxacin and gentamicin diffusion in Proteus mirabilis O18 biofilm by laser interferometry method. Acta Biochim Pol 60:707–711

    PubMed  Google Scholar 

  2. Danis-Wlodarczyk K, Olszak T, Arabski M et al (2015) Characterization of the newly isolated lytic bacteriophages KTN6 and KT28 and their efficacy against Pseudomonas aeruginosa biofilm. PLoS One 10(5):e0127603

    Article  PubMed  PubMed Central  Google Scholar 

  3. Danis-Wlodarczyk K, Vandenheuvel D, Jang HB et al (2016) A proposed integrated approach for the preclinical evaluation of phage therapy in Pseudomonas infections. Sci Rep 6:28115

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Arabski M, Wąsik S, Dworecki K et al (2007) Laser interferometric determination of ampicillin and colistin transfer through cellulose biomembrane in the presence of Proteus vulgaris O25 lipopolysaccharide. J Membr Sci 299:268–275

    Article  CAS  Google Scholar 

  5. Arabski M, Wąsik S, Dworecki K et al (2009) Laser interferometric and cultivation methods for measurement of colistin/ampicillin and saponin interactions with smooth and rough of Proteus mirabilis lipopolysaccharides and cells. J Microbiol Methods 77:179–183

    Article  Google Scholar 

  6. Arabski M, Davydova VN, Wąsik S et al (2009) Binding and biological properties of lipopolysaccharide Proteus vulgaris O25 (48/57)-chitosan complexes. Carbohydr Polym 78:481–487

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Michał Arabski .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Arabski, M., Wąsik, S. (2017). Laser Interferometry Method as a Novel Tool in Endotoxins Research. In: Holst, O. (eds) Microbial Toxins. Methods in Molecular Biology, vol 1600. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6958-6_12

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6958-6_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6956-2

  • Online ISBN: 978-1-4939-6958-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics