Abstract
Cryopreservation and storage of culture-expanded mesenchymal stem cells (MSCs) is essential for a biobank to maintain a collection of cell lines for research and clinical use. Optimization of cryopreservation protocols and methods to minimize damage to cells during freezing and thawing is critical to ensure reliable availability of viable cells. Controlling the freezing rate and the use of appropriate cryoprotectant, as well as stable storage temperature, can minimize the negative effects on cell viability. In this chapter, protocols for cryopreserving MSCs are described.
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References
Nicol A, Nieda M, Donaldson C et al (1996) Cryopreserved human bone marrow stroma is fully functional in vitro. Br J Haematol 94:258–265
Haack-Sorensen M, Bindslev L, Mortensen S et al (2007) The influence of freezing and storage on the characteristics and functions of human mesenchymal stromal cells isolated for clinical use. Cytotherapy 9:328–337
Ware CB, Baran SW (2007) A controlled-cooling protocol for cryopreservation of human and non-human primate embryonic stem cells. Methods Mol Biol 407:43–49
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Pavón, A., Beloqui, I., Salcedo, J.M., Martin, A.G. (2017). Cryobanking Mesenchymal Stem Cells. In: Crook, J., Ludwig, T. (eds) Stem Cell Banking. Methods in Molecular Biology, vol 1590. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6921-0_14
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DOI: https://doi.org/10.1007/978-1-4939-6921-0_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6919-7
Online ISBN: 978-1-4939-6921-0
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