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Label-Free Direct Detection of MiRNAs with Poly-Silicon Nanowire Biosensors

  • Jing He
  • Jianjun Zhu
  • Bin JiangEmail author
  • Yulan Zhao
Part of the Methods in Molecular Biology book series (MIMB, volume 1580)

Abstract

The diagnostic and prognostic value of microRNAs (miRNAs) in diseases becomes promising. Owing to fast response and high sensitivity, silicon nanowire (SiNW) biosensor has been considered a potential tool for miRNAs detection. Here, we describe a booming method to detect miRNAs with poly-silicon nanowire biosensors. Standard and real miRNA samples are applied in this study. The results show a limitation of 1 fM in the detection of standard miRNA sample with our poly-nanowire devices. Meanwhile, one-base mismatched sequence could be distinguished. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL.

Key words

miRNA SiNW 

Notes

Acknowledgment

The analysis was supported by National Natural Science Foundation of China (NSFC 30800401 and 81201604).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Shanghai Integrated Circuit Research & Development CenterShanghaiChina
  2. 2.School of Life ScienceEast China Normal UniversityShanghaiChina

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