Label-Free Direct Detection of MiRNAs with Poly-Silicon Nanowire Biosensors

  • Jing He
  • Jianjun Zhu
  • Bin JiangEmail author
  • Yulan Zhao
Part of the Methods in Molecular Biology book series (MIMB, volume 1580)


The diagnostic and prognostic value of microRNAs (miRNAs) in diseases becomes promising. Owing to fast response and high sensitivity, silicon nanowire (SiNW) biosensor has been considered a potential tool for miRNAs detection. Here, we describe a booming method to detect miRNAs with poly-silicon nanowire biosensors. Standard and real miRNA samples are applied in this study. The results show a limitation of 1 fM in the detection of standard miRNA sample with our poly-nanowire devices. Meanwhile, one-base mismatched sequence could be distinguished. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL.

Key words




The analysis was supported by National Natural Science Foundation of China (NSFC 30800401 and 81201604).


  1. 1.
    Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297CrossRefPubMedGoogle Scholar
  2. 2.
    Tay Y, Zhang J, Thomson AM, Lim B, Rigoutsos I (2008) MicroRNAs to Nanog, Oct4 and Sox2 coding regions modulate embryonic stem cell differentiation. Nature 455:1124–1128CrossRefPubMedGoogle Scholar
  3. 3.
    Guo D, Li Q, Lv Q, Wei Q, Cao S et al (2014) MiR-27a targets sFRP1 in hFOB cells to regulate proliferation, apoptosis and differentiation. PLoS One 9:e91354CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Chen X, Wang X, Ruan A, Han W, Zhao Y et al (2014) miR-141 Is a Key Regulator of Renal Cell Carcinoma Proliferation and Metastasis by Controlling EphA2 Expression. Clin Cancer Res 20:2617–2630CrossRefPubMedGoogle Scholar
  5. 5.
    Esquela-Kerscher A, Slack FJ (2006) Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer 6:259–269CrossRefPubMedGoogle Scholar
  6. 6.
    Lusis AJ (2000) Atherosclerosis. Nature 407:233–241CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Brase JC, Wuttig D, Kuner R, Sultmann H (2010) Serum microRNAs as non-invasive biomarkers for cancer. Mol Cancer 9:306CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Cortez MA, Bueso-Ramos C, Ferdin J, Lopez-Berestein G, Sood AK et al (2011) MicroRNAs in body fluids--the mix of hormones and biomarkers. Nat Rev Clin Oncol 8:467–477CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    de Planell-Saguer M, Rodicio MC (2013) Detection methods for microRNAs in clinic practice. Clin Biochem 46:869–878CrossRefPubMedGoogle Scholar
  10. 10.
    Zhang GJ, Chua JH, Chee RE, Agarwal A, Wong SM (2009) Label-free direct detection of MiRNAs with silicon nanowire biosensors. Biosens Bioelectron 24:2504–2508CrossRefPubMedGoogle Scholar
  11. 11.
    Dorvel BR, Reddy B Jr, Go J, Duarte Guevara C, Salm E et al (2012) Silicon nanowires with high-k hafnium oxide dielectrics for sensitive detection of small nucleic acid oligomers. ACS Nano 6:6150–6164CrossRefPubMedPubMedCentralGoogle Scholar

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© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Shanghai Integrated Circuit Research & Development CenterShanghaiChina
  2. 2.School of Life ScienceEast China Normal UniversityShanghaiChina

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