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MicroRNA Detection and Target Identification

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Tamas Dalmay

    1. School of Biological Sciences, University of East Anglia, Norwich, United Kingdom

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1580)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Improved Denaturation of Small RNA Duplexes and Its Application for Northern Blotting

    • C. Jake Harris, David C. Baulcombe, Attila Molnar
    Pages 1-6

  3. High-Throughput RT-qPCR for the Analysis of Circulating MicroRNAs

    • Geok Wee Tan, Lu Ping Tan
    Pages 7-19

  4. Genome-Wide Comparison of Next-Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum

    • Thorarinn Blondal, Maurizia Rossana Brunetto, Daniela Cavallone, Martin Mikkelsen, Michael Thorsen, Yuan Mang et al.
    Pages 21-44

  5. Small RNA Profiling by Next-Generation Sequencing Using High-Definition Adapters

    • Martina Billmeier, Ping Xu
    Pages 45-57

  6. Surface Acoustic Wave Lysis and Ion-Exchange Membrane Quantification of Exosomal MicroRNA

    • Katherine E. Richards, David B. Go, Reginald Hill
    Pages 59-70

  7. Droplet Microfluidic Device Fabrication and Use for Isothermal Amplification and Detection of MicroRNA

    • Maria Chiara Giuffrida, Roberta D’Agata, Giuseppe Spoto
    Pages 71-78

  8. Interrogation of Functional miRNA–Target Interactions by CRISPR/Cas9 Genome Engineering

    • Yale S. Michaels, Qianxin Wu, Tudor A. Fulga
    Pages 79-97

  9. Cell-Free Urinary MicroRNAs Expression in Small-Scale Experiments

    • Ludek Zavesky, Eva Jandakova, Radovan Turyna, Daniela Duskova, Lucie Langmeierova, Vit Weinberger et al.
    Pages 99-106

  10. Peptide-Based Isolation of Argonaute Protein Complexes Using Ago-APP

    • Judith Hauptmann, Gunter Meister
    Pages 107-116

  11. Predicting Functional MicroRNA-mRNA Interactions

    • Zixing Wang, Yin Liu
    Pages 117-126

  12. Computational and Experimental Identification of Tissue-Specific MicroRNA Targets

    • Raheleh Amirkhah, Hojjat Naderi Meshkin, Ali Farazmand, John E. J. Rasko, Ulf Schmitz
    Pages 127-147

  13. sRNAtoolboxVM: Small RNA Analysis in a Virtual Machine

    • Cristina Gómez-Martín, Ricardo Lebrón, Antonio Rueda, José L. Oliver, Michael Hackenberg
    Pages 149-174

  14. An Assessment of the Next Generation of Animal miRNA Target Prediction Algorithms

    • Thomas Bradley, Simon Moxon
    Pages 175-191

  15. The UEA Small RNA Workbench: A Suite of Computational Tools for Small RNA Analysis

    • Irina Mohorianu, Matthew Benedict Stocks, Christopher Steven Applegate, Leighton Folkes, Vincent Moulton
    Pages 193-224

  16. Prediction of miRNA–mRNA Interactions Using miRGate

    • Eduardo Andrés-León, Gonzalo Gómez-López, David G. Pisano
    Pages 225-237

  17. Detection of microRNAs Using Chip-Based QuantStudio 3D Digital PCR

    • Cristina Borzi, Linda Calzolari, Davide Conte, Gabriella Sozzi, Orazio Fortunato
    Pages 239-247

  18. MiRNA Quantitation with Microelectrode Sensors Enabled by Enzymeless Electrochemical Signal Amplification

    • Tanyu Wang, Gangli Wang, Didier Merlin, Emilie Viennois
    Pages 249-263

  19. A Robust Protocol to Quantify Circulating Cancer Biomarker MicroRNAs

    • Emma Bell, Hannah L. Watson, Shivani Bailey, Matthew J. Murray, Nicholas Coleman
    Pages 265-279

  20. MicroRNAs, Regulatory Networks, and Comorbidities: Decoding Complex Systems

    • Francesco Russo, Kirstine Belling, Anders Boeck Jensen, Flavia Scoyni, Søren Brunak, Marco Pellegrini
    Pages 281-295
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Keywords

About this book

This volume is a collection of miRNA detection and target identification protocols, detailing new developments in the traditional detection approaches such as northern blot, quantitative real-time PCR, array, next generation sequencing, and in situ hybridization. The chapters in MicroRNA Detection and Target Identification: Methods and Protocols  guide readers through novel approaches such as nanotechnology, microfluidics, based detection methods, analysis of serum and urinary, miRNAs as biomarkers, target identification and experimental approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical,MicroRNA Detection and Target Identification: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • School of Biological Sciences, University of East Anglia, Norwich, United Kingdom

    Tamas Dalmay

Bibliographic Information

  • Book Title: MicroRNA Detection and Target Identification

  • Book Subtitle: Methods and Protocols

  • Editors: Tamas Dalmay

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6866-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6864-0Published: 24 April 2017

  • Softcover ISBN: 978-1-4939-8322-3Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6866-4Published: 21 April 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 304

  • Number of Illustrations: 9 b/w illustrations, 75 illustrations in colour

  • Topics: Human Genetics

  • Industry Sectors: Biotechnology, Pharma

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