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Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree

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Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1560))

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Abstract

Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.

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Author information

Authors and Affiliations

  1. Department of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia, via A. Ferrata, 9, 27100, Pavia, Italy

    Vittorio Bertone, Eleonora Tarantola & Isabel Freitas

Authors
  1. Vittorio Bertone
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  2. Eleonora Tarantola
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  3. Isabel Freitas
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Corresponding author

Correspondence to Vittorio Bertone .

Editor information

Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Carlo Pellicciari

  2. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Marco Biggiogera

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Bertone, V., Tarantola, E., Freitas, I. (2017). Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6788-9_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6787-2

  • Online ISBN: 978-1-4939-6788-9

  • eBook Packages: Springer Protocols

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