Histochemistry of Single Molecules

Methods and Protocols

  • Carlo Pellicciari
  • Marco Biggiogera

Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Vital Histochemistry

  3. Lectin Histochemistry

    1. Front Matter
      Pages 91-91
    2. Gerrit Wolters-Eisfeld, Udo Schumacher
      Pages 121-132
    3. Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Martínez-Hernández, Concepción Ferrer, Luis Miguel Pastor
      Pages 133-144
  4. Protein Histochemistry

  5. Lipid Histochemistry

    1. Front Matter
      Pages 195-195
    2. Víctor Carriel, Fernando Campos, José Aneiros-Fernández, John A. Kiernan
      Pages 197-206
    3. Víctor Carriel, Antonio Campos, Miguel Alaminos, Stefania Raimondo, Stefano Geuna
      Pages 207-218

About this book


This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets.  It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA  fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.


Histology Nucleic acid detection Metabolic processes Optical and electron microscopy Histochemical techniques Chemical targets Cytochemical methods

Editors and affiliations

  • Carlo Pellicciari
    • 1
  • Marco Biggiogera
    • 2
  1. 1.Department of Biology and BiotechnologyUniversity of PaviaPaviaItaly
  2. 2.Department of Biology and BiotechnologyUniversity of PaviaPaviaItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6788-9
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6787-2
  • Online ISBN 978-1-4939-6788-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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