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A Histochemical Technique for the Detection of Annonaceous Acetogenins

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Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1560))

Abstract

Annonaceous acetogenins (ACGs) are molecules with carbon numbers C35–C37, usually with tetrahydrofuran and tetrahydropyran rings and one terminal γ-lactone (usually α,β-unsaturated), in a large aliphatic chain that is varyingly hydroxylated, acetoxylated or ketonized. ACGs have ecological functions as insecticides and are pharmacologically promising due to their cytotoxic and antitumoral properties. They are found in the seeds, leaves, roots, flowers and fruits of annonaceous plants and can be detected during isolation via thin-layer chromatography using Kedde’s reagent, which reacts with the unsaturated lactone. This chapter describes the location in situ of ACGs in fresh sections of annonaceous seeds using Kedde’s reagent.

The acetogenins are located in the idioblasts, in the endosperm and in the embryonic axis during differentiation. This method can aid in the detection of ACGs with a terminal unsaturated γ-lactone in organs and tissues.

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Acknowledgments

The authors thank Dr. Patricia Rivas Manzano and Biol. Carlos Tonatiuh Chavira Ramírez for technical assistance with the cryostat sectioning in the Laboratory of Tissue and Reproductive Biology, Faculty of Sciences, National Autonomous University of Mexico.

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Authors and Affiliations

  1. Facultad de Ciencias, Universidad Nacional Autónoma de México, Avenida Universidad N° 3000, Colonia Universidad Nacional Autónoma de México, Coyoacán, 04510, Ciudad de Mexico, Mexico

    Guillermo Laguna-Hernández & Alicia Enriqueta Brechú-Franco

  2. Instituto de Ciencias Biológicas, Universidad de Ciencias y Artes de Chiapas, None, Libramiento Norte Poniente 1150. Col. LajasMaciel, Tuxtla Gutiérrez, 29039, Chiapas, Mexico

    Iván De la Cruz-Chacón & Alma Rosa González-Esquinca

Authors
  1. Guillermo Laguna-Hernández
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  2. Alicia Enriqueta Brechú-Franco
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  3. Iván De la Cruz-Chacón
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  4. Alma Rosa González-Esquinca
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Corresponding author

Correspondence to Guillermo Laguna-Hernández .

Editor information

Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Carlo Pellicciari

  2. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Marco Biggiogera

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Laguna-Hernández, G., Brechú-Franco, A.E., De la Cruz-Chacón, I., González-Esquinca, A.R. (2017). A Histochemical Technique for the Detection of Annonaceous Acetogenins. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_25

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  • DOI: https://doi.org/10.1007/978-1-4939-6788-9_25

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6787-2

  • Online ISBN: 978-1-4939-6788-9

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