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CD95-Mediated Calcium Signaling

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CD95

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1557))

Abstract

Intracellular calcium signals regulate cell function and cell survival by controlling many processes. CD95 engagement results in distinct intracellular calcium signals that control the cell fate, apoptosis, or survival, depending on the ligand (membrane or soluble). Intracellular calcium determination is an exquisite readout to explore the molecular mechanisms elicited by CD95 engagement. The most widely applied methods for studying calcium signaling pathways use fluorescent indicators and imaging methods with fluorescence microscopy. This technical approach, however, requires many precautions that we discuss in this chapter.

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Acknowledgments

This work was supported by INSERM, INCa and Ligue contre le cancer (comité des Pyrénées Atlantiques).

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Authors and Affiliations

  1. INSERM U1218, 229 COURS DE L’ARGONNE, CS 61283, 33076, Bordeaux Cedex, France

    Mehdi Hammadi, Vanessa Delcroix, Anne-Marie Vacher, Thomas Ducret & Pierre Vacher

Authors
  1. Mehdi Hammadi
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  2. Vanessa Delcroix
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  3. Anne-Marie Vacher
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  4. Thomas Ducret
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  5. Pierre Vacher
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Corresponding author

Correspondence to Pierre Vacher .

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Editors and Affiliations

  1. ER440-OSS, Label INSERM, Universite de Rennes CLCC Eugene Marquis, RENNES, France

    Patrick Legembre

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Hammadi, M., Delcroix, V., Vacher, AM., Ducret, T., Vacher, P. (2017). CD95-Mediated Calcium Signaling. In: Legembre, P. (eds) CD95. Methods in Molecular Biology, vol 1557. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6780-3_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6780-3_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6778-0

  • Online ISBN: 978-1-4939-6780-3

  • eBook Packages: Springer Protocols

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