Skip to main content
  • Book
  • © 2017

CD95

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1557)

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
  2. CD95 Stimulation with CD95L and DISC Analysis

    • Matthieu Le Gallo, Patrick Legembre
    Pages 11-18
  3. Immunoprecipitation of Death Inducing Signaling Complex by Caspase-8

    • Andrei Alexandru Constantinescu, Aymeric Morlé, Olivier Micheau
    Pages 19-31
  4. In Vitro Evaluation of the Apoptosis Function in Human Activated T Cells

    • Aude Magerus-Chatinet, Frédéric Rieux-Laucat
    Pages 33-40
  5. Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition

    • Melissa Thomas, Patrick Legembre
    Pages 41-48
  6. Generation and Application of Bioluminescent CD95 Ligand Fusion Proteins

    • Isabell Lang, Juliane Kums, Harald Wajant
    Pages 63-77
  7. CD95-Mediated Calcium Signaling

    • Mehdi Hammadi, Vanessa Delcroix, Anne-Marie Vacher, Thomas Ducret, Pierre Vacher
    Pages 79-93
  8. CD95-Mediated Proton Regulation

    • Auréa Cophignon, Mallorie Poët, Michael Monet, Michel Tauc, Laurent Counillon
    Pages 95-102
  9. Study of the CD95-Mediated Non-apoptotic Signaling Pathway: PI3K

    • Amélie Fouqué, Patrick Legembre
    Pages 103-110
  10. Organelle Separation and Cell Signaling

    • Leandro Silva Da Costa, Damien Arnoult
    Pages 111-115
  11. Detection of S-Acylated CD95 by Acyl-Biotin Exchange

    • Aurelie Rossin, Anne-Odile Hueber
    Pages 189-198
  12. Exploration of Fas S-Nitrosylation by the Biotin Switch Assay

    • Ali Bettaieb, Catherine Paul, Stéphanie Plenchette
    Pages 199-206
  13. Method to Measure Sphingomyelin Synthase Activity Changes in Response to CD95L

    • Fatima Bilal, Michaël Pérès, Nathalie Andrieu-Abadie, Thierry Levade, Bassam Badran, Ahmad Daher et al.
    Pages 207-212

About this book

This volume provides the most commonly used methods and protocols to study the apoptotic and non-apoptotic roles of CD95. Chapters explore molecular, biochemical, cellular methods and animal models to in order to better understand the biological functions of this cytokine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, CD95: Methods and Protocols aims to foster original research on CD95/CD95L couple. 


Editors and Affiliations

  • ER440-OSS, Label INSERM, Universite de Rennes CLCC Eugene Marquis, RENNES, France

    Patrick Legembre

Bibliographic Information

  • Book Title: CD95

  • Book Subtitle: Methods and Protocols

  • Editors: Patrick Legembre

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6780-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6778-0Published: 18 January 2017

  • Softcover ISBN: 978-1-4939-8298-1Published: 15 July 2018

  • eBook ISBN: 978-1-4939-6780-3Published: 11 January 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 230

  • Number of Illustrations: 25 b/w illustrations, 38 illustrations in colour

  • Topics: Protein Science

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access