High-Throughput Techniques for DNA Methylation Profiling

  • Sophie Petropoulos
  • David Cheishvili
  • Moshe SzyfEmail author
Part of the Methods in Pharmacology and Toxicology book series (MIPT)


In this chapter, commonly used methods to assess the genome-wide DNA methylation status are reviewed and compared. The methods described in this chapter include enrichment-based method, Methylated DNA Immunoprecipitation (MeDIP), paired with microarray technology and next generation sequencing, and sodium bisulfate-based techniques including Infinium HumanMethylation450 BeadChip (Illumina 450 K) and Reduced Representation Bisulfite Sequencing (RRBS).

An overview of each protocol, including description as to why particular steps are required or critical, is outlined. Further, the protocols are compared and advantages and disadvantages of each are discussed.

Key words

DNA methylation Sodium bisulfite Methylated DNA immunoprecipitation (MeDIP) Infinium HumanMethylation450 BeadChip (Illumina 450 K) Reduced Representation Bisulfite Sequencing (RRBS) Microarray Next generation sequencing 



S.P. is supported by the Mats Sundin Fellowship in Developmental Health. D. C. is supported by fellowship from the Israel Cancer Research Foundation.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Sophie Petropoulos
    • 1
  • David Cheishvili
    • 2
  • Moshe Szyf
    • 2
    Email author
  1. 1.Department of Clinical Science, Intervention and Technology (CLINTEC)Karolinska InstitutetStockholmSweden
  2. 2.Department of Pharmacology and TherapeuticsMcGill University Medical SchoolMontrealCanada

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