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Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases

  • Book
  • © 2017

Overview

Editors:
  1. Barbara Stefanska

    1. Department of Nutrition Science, Purdue University, West Lafayette, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. David J. MacEwan

    1. Department of Molecular and Clinical Pharmacology, Institute of Translational Medicine University of Liverpool, Liverpool, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Explores epigenetics from the wide-angle view of epigenomics
  • Features detailed methods sections which include data analyses, troubleshooting, and feasibility in different experimental settings
  • Includes contributions from key researchers in the field

Part of the book series: Methods in Pharmacology and Toxicology (MIPT)

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Table of contents (11 protocols)

  1. Front Matter

    Pages i-xii
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  2. High-Throughput Techniques for DNA Methylation Profiling

    • Sophie Petropoulos, David Cheishvili, Moshe Szyf
    Pages 1-15

  3. Reduced Representation Bisulfite Sequencing (RRBS) and Cell Sorting Prior to DNA Methylation Analysis in Psychiatric Disorders

    • Wilfred C. de Vega, Atif Hussain, Patrick O. McGowan
    Pages 17-32

  4. Targeted DNA Methylation Analysis Methods

    • David Cheishvili, Sophie Petropoulos, Steffan Christiansen, Moshe Szyf
    Pages 33-50

  5. Analyzing Targeted Nucleosome Position and Occupancy in Cancer, Obesity, and Diabetes

    • Prasad P. Devarshi, Tara M. Henagan
    Pages 51-62

  6. Synthesis and Application of Cell-Permeable Metabolites for Modulating Chromatin Modifications Regulated by α-Ketoglutarate-Dependent Enzymes

    • Hunter T. Balduf, Antonella Pepe, Ann L. Kirchmaier
    Pages 63-79

  7. High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR

    • Emily C. Dykhuizen, Leigh C. Carmody, Nicola J. Tolliday
    Pages 81-95

  8. Methods for MicroRNA Profiling in Cancer

    • Sushuma Yarlagadda, Anusha Thota, Ruchi Bansal, Jason Kwon, Murray Korc, Janaiah Kota
    Pages 97-113

  9. Microbiota and Epigenetic Regulation of Inflammatory Mediators

    • Marlene Remely, Heidrun Karlic, Irene Rebhan, Martina Greunz, Alexander G. Haslberger
    Pages 115-134

  10. Optical Microscopy and Spectroscopy for Epigenetic Modifications in Single Living Cells

    • Yi Cui, Joseph Irudayaraj
    Pages 135-154

  11. MicroRNAs in Therapy and Toxicity

    • David J. MacEwan, Niraj M. Shah, Daniel J. Antoine
    Pages 155-167

  12. Genetics and Epigenetics of Multiple Sclerosis

    • Borut Peterlin, Ales Maver, Vidmar Lovro, Luca Lovrečić
    Pages 169-192

  13. Back Matter

    Pages 193-197
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Keywords

About this book

This practical collection examines methodologies originating from the benefits of genome-wide approaches to studying epigenetics, which has opened the emerging field of epigenomics. Focusing on the areas of cancer, inflammatory and autoimmune disorders, chapters discuss three main components of the epigenome and their role in the regulation of gene expression and present a detailed method section specific to studying each component, including data analyses, troubleshooting, and feasibility in different experimental settings. The main topics are high-throughput and targeted methods for DNA methylation analysis, nucleosome position mapping, studying epigenetic effects of gut microbiota, optical imaging for detection of epigenetic aberrations in living cells, methods for microRNA, and histone code profiling. Written for the Methods in Pharmacology and Toxicology series, the book includes the kind of detail and implementation advice to encourage success in the lab. 
Authoritative and easily applicable, Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases aims to provide pharmacologists, molecular biologists, bioinformaticians, and toxicologists with a vital background on epigenetics and state-of-the-art techniques in epigenomics.

Editors and Affiliations

  • Department of Nutrition Science, Purdue University, West Lafayette, USA

    Barbara Stefanska

  • Department of Molecular and Clinical Pharmacology, Institute of Translational Medicine University of Liverpool, Liverpool, United Kingdom

    David J. MacEwan

Bibliographic Information

  • Book Title: Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases

  • Editors: Barbara Stefanska, David J. MacEwan

  • Series Title: Methods in Pharmacology and Toxicology

  • DOI: https://doi.org/10.1007/978-1-4939-6743-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-6741-4Published: 14 December 2016

  • Softcover ISBN: 978-1-4939-8289-9Published: 07 July 2018

  • eBook ISBN: 978-1-4939-6743-8Published: 12 December 2016

  • Series ISSN: 1557-2153

  • Series E-ISSN: 1940-6053

  • Edition Number: 1

  • Number of Pages: XII, 197

  • Number of Illustrations: 16 b/w illustrations, 22 illustrations in colour

  • Topics: Pharmacology/Toxicology

  • Industry Sectors: Biotechnology, Chemical Manufacturing, Consumer Packaged Goods, Health & Hospitals, Pharma

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