Abstract
Primary cells that reach the end of their replicative potential, encounter sublethal stress, or experience the activation of certain oncogenes cease proliferation and enter a state of long-term growth arrest named senescence. The senescent process has been implicated in a variety of age-related diseases and also in the pathogenesis of cancer. Senescence is characterized by distinct changes in the types and levels of coding RNAs (mRNAs) as well as in the vast collective of regulatory noncoding (nc)RNAs, which includes microRNAs, long noncoding RNAs (lncRNAs), and circular (circRNAs). Numerous technologies permit the detection of senescence-associated linear transcripts (mRNAs, lncRNAs, microRNAs), but the identification and quantification of circRNAs in senescence require distinct molecular approaches. In this chapter, we describe a method for the detection and measurement of circRNAs in senescent cells using specialized reverse transcription (RT) followed by real-time quantitative (q)PCR analysis.
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Acknowledgments
This work was supported in its entirety by the National Institute on Aging Intramural Research Program of the National Institutes of Health.
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Panda, A.C., Abdelmohsen, K., Gorospe, M. (2017). RT-qPCR Detection of Senescence-Associated Circular RNAs. In: Nikiforov, M. (eds) Oncogene-Induced Senescence. Methods in Molecular Biology, vol 1534. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6670-7_7
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DOI: https://doi.org/10.1007/978-1-4939-6670-7_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6668-4
Online ISBN: 978-1-4939-6670-7
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