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Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo

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Tau Protein

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1523))

Abstract

Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.

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Acknowledgment

We thank the Kayed Lab members for their contributions to the optimization of the protocols and useful suggestions. This work was supported by the Mitchell Center for Neurodegenerative Diseases.

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Authors and Affiliations

  1. George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX, 77555, USA

    Julia E. Gerson, Urmi Sengupta & Rakez Kayed Ph.D.

  2. Departments of Neurology, and Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555-1045, USA

    Julia E. Gerson & Urmi Sengupta

  3. Departments of Neurology, and Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555, USA

    Rakez Kayed Ph.D.

  4. University of Texas Medical Branch, 301 University Boulevard, Medical Research Building, Room 10.138C, Galveston, TX, 77555-1045, USA

    Rakez Kayed Ph.D.

Authors
  1. Julia E. Gerson
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  2. Urmi Sengupta
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  3. Rakez Kayed Ph.D.
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Corresponding author

Correspondence to Rakez Kayed Ph.D. .

Editor information

Editors and Affiliations

  1. UMR CNRS 8576, Villeneuve d’Ascq, France

    Caroline Smet-Nocca

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Gerson, J.E., Sengupta, U., Kayed, R. (2017). Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo. In: Smet-Nocca, C. (eds) Tau Protein. Methods in Molecular Biology, vol 1523. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6598-4_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6598-4_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6596-0

  • Online ISBN: 978-1-4939-6598-4

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