Tau Protein

Methods and Protocols

  • Caroline Smet-Nocca

Part of the Methods in Molecular Biology book series (MIMB, volume 1523)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Native Tau Protein: Conformational Studies and Tubulin Interactions

    1. Front Matter
      Pages 1-1
    2. Nalini Vijay Gorantla, Alexander V. Shkumatov, Subashchandrabose Chinnathambi
      Pages 3-20
    3. Nalini Vijay Gorantla, Puneet Khandelwal, Pankaj Poddar, Subashchandrabose Chinnathambi
      Pages 21-31
    4. Tiphany De Bessa, Gilles Breuzard, Diane Allegro, François Devred, Vincent Peyrot, Pascale Barbier
      Pages 61-85
  3. In Vitro Self-Association of Tau Protein: Oligomer and Fibril Formation

  4. Tau Post-Translational Modifications

    1. Front Matter
      Pages 159-159
    2. Oliver Reimann, Caroline Smet-Nocca, Christian P. R. Hackenberger
      Pages 215-235
  5. Detecting Tau Proteins, Their Modifications, Mutations and Interacting Partners

    1. Front Matter
      Pages 249-249
    2. Nicolas Sergeant, Francisco-Jose Fernandez-Gomez, Helene Obriot, Sabiha Eddarkaoui, Valérie Buée-Scherrer, Luc Buée
      Pages 251-261
    3. Franck R. Petry, Samantha B. Nicholls, Sébastien S. Hébert, Emmanuel Planel
      Pages 263-272

About this book

Introduction

This detailed volume gathers basic and advanced methods and protocols from in vitro assays and in vivo models to address the molecular and functional aspects of tau physiopathology. Divided into five parts that illustrate the underlying molecular mechanisms of Tau functions and dysfunctions in Alzheimer’s disease and other dementia referred to as tauopathies, this volume discusses timely topics such as: conformational studies of native tau protein and investigation of its physiological function in microtubules binding and tubulin polymerization; in vitro methods of formation and detection of tau oligomers and PHF-like fibrils; characterization and in vitro introduction of post-translational modifications on tau proteins for further functional studies; analytical tools for the detection of tau proteins, their modifications and cellular interactions, and MAPT gene mutations in various biological samples; and cellular and in vivo models for the investigations of tau physiopathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and cutting-edge, Tau Protein: Methods and Protocols is a valuable resource that addresses the most recent aspects of Alzheimer’s disease research related to Tau protein and state-of-the-art experimental techniques. It targets a broad scientific audience including molecular biologists and biochemists, as well as researchers interested in exploring the functions of tau proteins. 

Keywords

tau physiopathology microtubule binding tubulin polymerization formation of tau oligomers PHF-like fibrils fibrillization kinetics screening assay of tau aggregation inhibitors MAPT gene mutations

Editors and affiliations

  • Caroline Smet-Nocca
    • 1
  1. 1.UMR CNRS 8576Villeneuve d’AscqFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6598-4
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6596-0
  • Online ISBN 978-1-4939-6598-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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