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Tissue Engineering Platforms to Replicate the Tumor Microenvironment of Multiple Myeloma

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Cancer Gene Networks

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1513))

Abstract

We described here the manufacturing and implementation of two prototype perfusion culture devices designed primarily for the cultivation of difficult-to-preserve primary patient-derived multiple myeloma cells (MMC). The first device consists of an osteoblast (OSB)-derived 3D tissue scaffold constructed in a perfused microfluidic environment. The second platform is a 96-well plate-modified perfusion culture device that can be utilized to reconstruct several tissue and tumor microenvironments utilizing both primary human and murine cells. This culture device was designed and fabricated specifically to: (1) enable the preservation of primary MMC for downstream use in biological studies and chemosensitivity analyses and, (2) provide a high-throughput format that is compatible with plate readers specifically seeing that this system is built on an industry standard 96-well tissue culture plate.

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Acknowledgements

Woo Y. Lee and Jenny Zilberberg are equal contributors in this work. We thank Dr. David Siegel at HUMC for providing MM patient biospecimens and Dr. Peter Tolias at Stevens for useful discussions. This work was supported in part by the Provost Office of the Stevens Institute of Technology (Stevens), the John Theurer Cancer Center at Hackensack University Medical Center (HackensackUMC), and the National Institutes of Health grants (1R21CA174543 to J.Z. and W.Y.L.).

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Authors and Affiliations

  1. Department of Chemical Engineering and Materials Science, Stevens Institute of Technology, Hoboken, NJ, 07030, USA

    Wenting Zhang & Woo Y. Lee

  2. John Theurer Cancer Center, Hackensack University Medical Center, 40 Prospect Avenue, Hackensack, NJ, 07601, USA

    Jenny Zilberberg

Authors
  1. Wenting Zhang
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  2. Woo Y. Lee
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  3. Jenny Zilberberg
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Corresponding author

Correspondence to Jenny Zilberberg .

Editor information

Editors and Affiliations

  1. Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, District of Columbia, USA

    Usha Kasid

  2. Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, District of Columbia, USA

    Robert Clarke

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Zhang, W., Lee, W.Y., Zilberberg, J. (2017). Tissue Engineering Platforms to Replicate the Tumor Microenvironment of Multiple Myeloma. In: Kasid, U., Clarke, R. (eds) Cancer Gene Networks. Methods in Molecular Biology, vol 1513. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6539-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6539-7_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6537-3

  • Online ISBN: 978-1-4939-6539-7

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