Tissue Engineering Platforms to Replicate the Tumor Microenvironment of Multiple Myeloma
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We described here the manufacturing and implementation of two prototype perfusion culture devices designed primarily for the cultivation of difficult-to-preserve primary patient-derived multiple myeloma cells (MMC). The first device consists of an osteoblast (OSB)-derived 3D tissue scaffold constructed in a perfused microfluidic environment. The second platform is a 96-well plate-modified perfusion culture device that can be utilized to reconstruct several tissue and tumor microenvironments utilizing both primary human and murine cells. This culture device was designed and fabricated specifically to: (1) enable the preservation of primary MMC for downstream use in biological studies and chemosensitivity analyses and, (2) provide a high-throughput format that is compatible with plate readers specifically seeing that this system is built on an industry standard 96-well tissue culture plate.
Key wordsMicroenvironment Three-dimensional Microfluidics Perfusion Multiple myeloma
Woo Y. Lee and Jenny Zilberberg are equal contributors in this work. We thank Dr. David Siegel at HUMC for providing MM patient biospecimens and Dr. Peter Tolias at Stevens for useful discussions. This work was supported in part by the Provost Office of the Stevens Institute of Technology (Stevens), the John Theurer Cancer Center at Hackensack University Medical Center (HackensackUMC), and the National Institutes of Health grants (1R21CA174543 to J.Z. and W.Y.L.).
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