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Plant Hormones pp 249-258 | Cite as

Hormone Profiling in Plant Tissues

  • Maren Müller
  • Sergi Munné-BoschEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1497)

Abstract

Plant hormones are for a long time known to act as chemical messengers in the regulation of physiological processes during a plant’s life cycle, from germination to senescence. Furthermore, plant hormones simultaneously coordinate physiological responses to biotic and abiotic stresses. To study the hormonal regulation of physiological processes, three main approaches have been used (1) exogenous application of hormones, (2) correlative studies through measurements of endogenous hormone levels, and (3) use of transgenic and/or mutant plants altered in hormone metabolism or signaling. A plant hormone profiling method is useful to unravel cross talk between hormones and help unravel the hormonal regulation of physiological processes in studies using any of the aforementioned approaches. However, hormone profiling is still particularly challenging due to their very low abundance in plant tissues. In this chapter, a sensitive, rapid, and accurate method to quantify all the five “classic” classes of plant hormones plus other plant growth regulators, such as jasmonates, salicylic acid, melatonin, and brassinosteroids is described. The method includes a fast and simple extraction procedure without time consuming steps as purification or derivatization, followed by optimized ultrahigh-performance liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (UHPLC-MS/MS) analysis. This protocol facilitates the high-throughput analysis of hormone profiling and is applicable to different plant tissues.

Key words

Plant hormones UPLC/ESI-MS/MS Auxin Abscisic acid Brassinosteroids Cytokinins Ethylene Gibberellins Jasmonates Melatonin Salicylic acid 

Notes

Acknowledgments

We thank Barbara Simancas and Javier A. Miret for help in extraction of Arabidopsis thaliana leaves and Iris × hollandica tepals.

References

  1. 1.
    Pan X, Welti R, Wang X (2008) Simultaneous quantification of major phytohormones and related compounds in crude plant extracts by liquid chromatography tandem mass spectrometry. Phytochemistry 69:1773–1781CrossRefPubMedGoogle Scholar
  2. 2.
    Zhang N, Sun Q, Zhang H, Cao Y, Weeda S, Ren S, Guo Y-D (2015) Roles of melatonin in abiotic stress resistance in plants. J Exp Bot 66:647–656CrossRefPubMedGoogle Scholar
  3. 3.
    Davies PJ (2010) The plant hormones: their nature, occurrence, and functions. Kluwer Academic, The NetherlandsCrossRefGoogle Scholar
  4. 4.
    Farnsworth E (2004) Hormones and shifting ecology throughout plant development. Ecology 85:5–15CrossRefGoogle Scholar
  5. 5.
    Van Meulenbrock L, Vanden BJ, Steppe K et al (2012) Ultra-high performance liquid chromatography coupled to high resolution Orbitrab mass spectrometry for metabolomics profiling of the endogenous phytohormonal status of the tomato plant. J Chromatogr A 1260:67–80CrossRefGoogle Scholar
  6. 6.
    Baiguz A, Tretyn A (2003) The chemical characteristic and distribution of brassinosteroids in plants. Phytochemistry 62:1027–1046CrossRefGoogle Scholar
  7. 7.
    Takatsu S (1994) Brassinosteroids: distribution in plants, bioassays and microanalysis by gas chromatography-mass spectrometry. J Chromatogr A 658:3–15CrossRefGoogle Scholar
  8. 8.
    Müller A, Düchting P, Weiler EW (2002) A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana. Planta 216:44–56CrossRefPubMedGoogle Scholar
  9. 11.
    Müller M, Munné-Bosch S (2011) Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Plant Methods 7:37CrossRefPubMedPubMedCentralGoogle Scholar
  10. 12.
    Almeida Trapp M, De Souza GD, Rodrigues-Filho E et al (2014) Validated method for phytohomone quantification in plants. Front Plant Sci 5:417CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Departament de Biologia Vegetal, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain

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