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Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy

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Germline Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1463))

Abstract

The Drosophila ovary represents a key in vivo model used to study germline stem cell (GSC) maintenance and stem cell daughter differentiation because these cells and their somatic cell neighbors can be identified at single-cell resolution within their native environment. Here we describe a fluorescent-based technique for the acquisition of 4D datasets of the Drosophila ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the investigation of molecular and cellular dynamics that were not previously possible using still images.

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References

  1. Eliazer S, Buszczak M (2011) Finding a niche: studies from the Drosophila ovary. Stem Cell Res Ther 2:45

    Article  PubMed  PubMed Central  Google Scholar 

  2. Gilboa L (2015) Organizing stem cell units in the Drosophila ovary. Curr Opin Genet Dev 32:31–36

    Article  CAS  PubMed  Google Scholar 

  3. Slaidina M, Lehmann R (2014) Translational control in germline stem cell development. J Cell Biol 207:13–21

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Fichelson P, Moch C, Ivanovitch K et al (2009) Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila. Nat Cell Biol 11:685–693

    Article  CAS  PubMed  Google Scholar 

  5. Zhang Q, Shalaby NA, Buszczak M (2014) Changes in rRNA transcription influence proliferation and cell fate within a stem cell lineage. Science 343:298–301

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Nienhaus GU, Nienhaus K, Holzle A et al (2006) Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications. Photochem Photobiol 82:351–358

    Article  CAS  PubMed  Google Scholar 

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Acknowledgement

We would like to thank P. Robin Hiesinger and Neset Ozel for their input and advice while developing this protocol.

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Authors and Affiliations

  1. Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA

    Nevine A. Shalaby & Michael Buszczak

  2. Institute for Biology, Freie Universität Berlin, 14195, Berlin, Germany

    Nevine A. Shalaby

Authors
  1. Nevine A. Shalaby
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  2. Michael Buszczak
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Corresponding authors

Correspondence to Nevine A. Shalaby or Michael Buszczak .

Editor information

Editors and Affiliations

  1. Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA

    Michael Buszczak

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Shalaby, N.A., Buszczak, M. (2017). Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy. In: Buszczak, M. (eds) Germline Stem Cells. Methods in Molecular Biology, vol 1463. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4017-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-4017-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-4015-8

  • Online ISBN: 978-1-4939-4017-2

  • eBook Packages: Springer Protocols

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