Abstract
During the past several years, the human placenta and in particular the amniotic fetal membrane have attracted much attention as a possible source of cells to be used in cell therapy approaches due to its putative stem cell potential associated with its early embryonic origin and its immunomodulatory potential associated with its role in fetomaternal tolerance.
Within the human amniotic membrane, it is possible to isolate two main cell populations: amniotic epithelial cells (from the epithelial layer of the amniotic membrane) and amniotic mesenchymal cells (from the mesenchymal layer of the amniotic membrane). In this chapter, we will describe a method for the isolation of mesenchymal stromal cells (hAMSCs). We will also describe their optimal culture conditions and the phenotypic characterization of cells after passaging.
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Acknowledgments
The authors would like to thank all the CREM personnel who contributed to the optimization of these protocols.
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Magatti, M., Pianta, S., Silini, A., Parolini, O. (2016). Isolation, Culture, and Phenotypic Characterization of Mesenchymal Stromal Cells from the Amniotic Membrane of the Human Term Placenta. In: Gnecchi, M. (eds) Mesenchymal Stem Cells. Methods in Molecular Biology, vol 1416. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3584-0_13
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DOI: https://doi.org/10.1007/978-1-4939-3584-0_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3582-6
Online ISBN: 978-1-4939-3584-0
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