Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1416)
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Table of contents (33 protocols)
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Front Matter
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Overview of Mesenchymal Stem Cells For Cell Therapy
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Front Matter
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Isolation and Characterization of Mesenchymal Stem Cells
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Front Matter
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Keywords
About this book
This volume aims to outline the current status of the Mesenchymal Stem Cells(MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than ever, “under the spotlight”. Mesenchymal Stem Cells: Methods and Protocols, Second Edition is organized into four sections. The first guides the reader through a series of state-of-the-art reviews summarizing the use of MSC for the treatment of various diseases. The other three sections are a collection of methodological chapters covering several aspects: isolation and characterization of MSC; expansion of MSC for clinical use; production and characterization of the MSC secretome. Written in the highly successful Methods in Molecular Biology series format, the method chapters include introductions to their respective topics, complete lists of the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and tips on troubleshooting which will help the researcher to avoid known pitfalls.
Authoritative and cutting-edge, Mesenchymal Stem Cells: Methods and Protocols, Second Edition, aims to ensure successful results in the further study of this vital field.
Reviews
“‘Mesenchymal Stem Cells: Methods and Protocols’ represents an outstanding comprehensive work helping scientists to understand better the role of MSC and its secretome in regenerative medicine. … The book should not be missed in all biomedical research laboratories interested in the development of clinically compliant manufacturing of therapeutic MSC or their derivatives. The book provides a great help to PhD students and postdocs involved in the rapidly developing research field of personified medicine.” (Cestmir Altaner, Neoplasma, Vol. 63 (5), 2016)
Editors and Affiliations
Bibliographic Information
Book Title: Mesenchymal Stem Cells
Book Subtitle: Methods and Protocols
Editors: Massimiliano Gnecchi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3584-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3582-6Published: 29 May 2016
Softcover ISBN: 978-1-4939-8085-7Published: 26 May 2018
eBook ISBN: 978-1-4939-3584-0Published: 28 May 2016
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIX, 570
Number of Illustrations: 10 b/w illustrations, 14 illustrations in colour
Topics: Cell Biology
Industry Sectors: Biotechnology, Pharma