Abstract
Black pepper, Piper nigrum L., the “King of spices” is the most widely used spice growing in the South-Western region of India. The humid tropical evergreen forest bordering the Malabar Coast (Western Ghats is one of the hot spot areas of plant bio-diversity on earth) is its center of origin and diversity. However, the crop faces constraints like rampant fungal and viral diseases, lack of disease free planting material, hence biotechnological tools can be utilized to address these problems and strides have been made successfully. The standardization of micropropagation, somatic embryogenesis, in vitro conservation, protoplast isolation, and genetic transformation protocols are described here. The protocols could be utilized to achieve similar goals in the related species of Piper too.
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Abbreviations
- BA:
-
Benzyl adenine
- IBA :
-
Indole-3-butyric acid
- Kin:
-
Kinetin
- MS:
-
Murashige and Skoog
- NAA :
-
α-naphthalene acetic acid
- SH:
-
Schenk and Hildebrandt
- TDZ :
-
Thiaduzuron
- WPM:
-
Woody plant medium
References
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Nirmal Babu, K., Divakaran, M., Yamuna, G., Ravindran, P.N., Peter, K.V. (2016). Protocols for Improvement of Black Pepper (Piper nigrum L.) Utilizing Biotechnological Tools. In: Jain, S. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition. Methods in Molecular Biology, vol 1391. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3332-7_26
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DOI: https://doi.org/10.1007/978-1-4939-3332-7_26
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3330-3
Online ISBN: 978-1-4939-3332-7
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