2-DE Gel Analysis: The Spot Detection

  • Simona MartinottiEmail author
  • Elia Ranzato
Part of the Methods in Molecular Biology book series (MIMB, volume 1384)


The abundance of different proteins on a 2-DE gel is reflected by the shape, size, and intensity of the corresponding spots. Protein quantitation requires the conversion of an analog gel image into digital data, resulting into a catalog of individual spots listed as x, y positions, shape parameters, and quantitative values. So, it is possible to carry out objective comparisons of equivalent spots on different gels, determining whether a particular protein is more or less abundant in one sample compared with another.

Unfortunately, spots on protein gels are not uniform in shape, size, or density, and detection, quantitation, and comparison can be challenging without intervention.

Once a processed image is available, a number of different algorithms can be applied to detect and quantitate individual spots.

Key words

Spot detection Spot segmentation Image analysis 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.DiSIT—Dipartimento di Scienze e Innovazione TecnologicaUniversity of Piemonte OrientaleAlessandriaItaly

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