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2-DE Gel Analysis: The Spot Detection

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2-D PAGE Map Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1384))

Abstract

The abundance of different proteins on a 2-DE gel is reflected by the shape, size, and intensity of the corresponding spots. Protein quantitation requires the conversion of an analog gel image into digital data, resulting into a catalog of individual spots listed as x, y positions, shape parameters, and quantitative values. So, it is possible to carry out objective comparisons of equivalent spots on different gels, determining whether a particular protein is more or less abundant in one sample compared with another.

Unfortunately, spots on protein gels are not uniform in shape, size, or density, and detection, quantitation, and comparison can be challenging without intervention.

Once a processed image is available, a number of different algorithms can be applied to detect and quantitate individual spots.

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Author information

Authors and Affiliations

  1. DiSIT—Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, viale Teresa Michel, 11, Alessandria, 15121, Italy

    Simona Martinotti & Elia Ranzato

Authors
  1. Simona Martinotti
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  2. Elia Ranzato
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Corresponding author

Correspondence to Simona Martinotti .

Editor information

Editors and Affiliations

  1. Department of Sciences and Technological Innovation, Università del Piemonte Orientale, Alessandria, Italy

    Emilio Marengo

  2. Department of Sciences and Technological Innovation, Università del Piemonte Orientale , Alessandria, Italy

    Elisa Robotti

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Martinotti, S., Ranzato, E. (2016). 2-DE Gel Analysis: The Spot Detection. In: Marengo, E., Robotti, E. (eds) 2-D PAGE Map Analysis. Methods in Molecular Biology, vol 1384. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3255-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3255-9_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3254-2

  • Online ISBN: 978-1-4939-3255-9

  • eBook Packages: Springer Protocols

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