2-D PAGE Map Analysis

Methods and Protocols

  • Emilio Marengo
  • Elisa Robotti

Part of the Methods in Molecular Biology book series (MIMB, volume 1384)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. 2D-Maps Reproducibility and Maps Modeling

    1. Front Matter
      Pages 1-1
    2. Maria Chiara Pietrogrande, Nicola Marchetti, Francesco Dondi
      Pages 39-53
  3. Image Analysis Tools to Provide Spot Volume Datasets

    1. Front Matter
      Pages 67-67
    2. Elisa Robotti, Emilio Marengo, Fabio Quasso
      Pages 91-107
    3. Marcello Manfredi, Elisa Robotti, Emilio Marengo
      Pages 119-154
    4. Simona Martinotti, Elia Ranzato
      Pages 155-164
    5. Jeffrey S. Morris, Howard B. Gutstein
      Pages 185-201
    6. Massimo Natale, Alfonso Caiazzo, Elisa Ficarra
      Pages 203-211
  4. Statistical Methods Applied to Spot Volume Datasets to Identify Candidate Biomarkers

    1. Front Matter
      Pages 213-213
    2. Sebastien C. Carpentier
      Pages 215-235
  5. Differential Analysis from Direct Image Analysis Tools

    1. Front Matter
      Pages 269-269
    2. Emilio Marengo, Elisa Robotti, Marco Demartini
      Pages 271-288

About this book

Introduction

Exploring the 2-D gel mapping field, the chapters in this book are separated into four different categories: Part I talks about 2-D maps reproducibility and maps modeling; Part II describes the image analysis tools that provide spot volume datasets; Part III is about the statistical methods applied to spot volume datasets to identify candidate biomarkers; and Part IV discusses differential analysis from direct image analysis tools. 2-D PAGE Map Analysis: Methods and Protocols provides a unique approach to 2-D gel mapping, in that it helps users avoid drawbacks due to ignorance of the basic theoretical mechanisms underlying the technique, including data handling and proper tools for spot analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, 2-D PAGE Map Analysis: Methods and Protocols, is a useful resource for any scientist or researcher, with a mathematical background, who is interested in 2-D gel mapping.

Keywords

bioinformatics tools complex protein extracts gel image isoelectric focusing spot detection spot volume datasets staining two-dimensional polyacrylamide gel-electrophoresis

Editors and affiliations

  • Emilio Marengo
    • 1
  • Elisa Robotti
    • 2
  1. 1.Department of Sciences and Technological InnovationUniversità del Piemonte OrientaleAlessandriaItaly
  2. 2.Department of Sciences and Technological InnovationUniversità del Piemonte Orientale AlessandriaItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3255-9
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3254-2
  • Online ISBN 978-1-4939-3255-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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