Abstract
There is a huge potential interest to use synthetic polymers as versatile solid supports for analytical microarraying. Chemical modification of polycarbonate (PC) for covalent immobilization of probes, micro-printing of protein or nucleic acid probes, development of indirect immunoassay, and development of hybridization protocols are described and discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hook DG, AAnderson DG, Langer R, Williams P, Davies MC, Alexander MR (2010) High throughput methods applied in biomaterial development and discovery. Biomaterials 31:187–198
Bhattacharyya D, Fakirov S (2012) Synthetic polymer–polymer composites. Carl Hanser Verlag, Munich, Germany
Tamarit-López J, Morais S, Puchades R, Maquieira A (2011) Oxygen plasma treated interactive polycarbonate DNA microarraying platform. Bioconjug Chem 22:2573–2580
Campàs M, Katakis I (2004) DNA biochip arraying, detection and amplification strategies. Trends Anal Chem 23:49–62
Miller MB, Tang Y-W (2009) Basic concepts of microarrays and potential applications in clinical microbiology. Clin Microbiol Rev 22:611–633
Bañuls MJ, García-Piñón F, Puchades R, Maquieira A (2008) Chemical derivatization of compact disc polycarbonate surfaces for SNPs detection. Bioconjug Chem 19:665–672
Tamarit-López J, Morais S, Bañuls MJ, Puchades R, Maquieira A (2010) Development of hapten-linked microimmunoassays on polycarbonate discs. Anal Chem 82:1954–1963
Morais S, Tamarit Lopez J, Carrascosa J, Puchades R, Maquieira A (2008) Analytical prospect of compact disk technology in immunosensing. Anal Bioanal Chem 391:2837–2844
Arnandis-Chover T, Morais S, Tortajada LA, Puchades R, Maquieira A, Olabarria G, Berganza J (2012) Detection of food-borne pathogens with DNA arrays on disk. Talanta 101:405–412
Vazquez J, Qushair G, Albericio F (2003) Qualitative colorimetric tests for solid phase synthesis. Methods Enzymol 369:21–27
Xu F, Datta P, Wang H, Gurung S, Hashimoto M, Wei S, Goettert J, McCarley RL, Soper SA (2007) Polymer microfluidic chips with integrated waveguides for reading microarrays. Anal Chem 79:9007–9013
Zubritsky E (2000) Spotting a microarray system. Anal Chem 72:761–767
Morais S, Tortajada-Genaro LA, Arnandis-Chover T, Puchades R, Maquieira A (2009) Multiplexed microimmunoassays on a Digital Versatile Disk. Anal Chem 81:5646–5654
McWilliam I, Kwan MC, Hall D (2011) Inkjet printing for the production of protein microarrays. In Protein Microarrays. Methods and Protocols. Korf U (Ed.). 345–361. Humana Press
Acknowledgements
This research has been funded through FEDER Projects: GVA-PROMETEO II/2014/040 (Generalitat Valenciana) and MINECO CTQ/2013-45875-R.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Bañuls, MJ., Morais, S.B., Tortajada-Genaro, L.A., Maquieira, Á. (2016). Microarray Developed on Plastic Substrates. In: Li, P., Sedighi, A., Wang, L. (eds) Microarray Technology. Methods in Molecular Biology, vol 1368. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3136-1_4
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3136-1_4
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3135-4
Online ISBN: 978-1-4939-3136-1
eBook Packages: Springer Protocols