Abstract
Paramyxoviruses are able to stably express a wide-variety of heterologous antigens at relatively high levels in various species and are consequently considered as potent gene delivery vehicles. A single vaccination is frequently sufficient for the induction of robust humoral and cellular immune responses. Here we provide detailed methods for the construction and application of Newcastle disease virus (NDV)-based vector vaccines. The in silico design and in vitro rescue as well as the in vivo evaluation of NDV based vectors are described in this chapter.
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Dr. Jeroen Kortekaas is acknowledged for carefully reading of the protocol.
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Wichgers Schreur, P.J. (2016). Construction and Application of Newcastle Disease Virus-Based Vector Vaccines. In: Brun, A. (eds) Vaccine Technologies for Veterinary Viral Diseases. Methods in Molecular Biology, vol 1349. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3008-1_14
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DOI: https://doi.org/10.1007/978-1-4939-3008-1_14
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3007-4
Online ISBN: 978-1-4939-3008-1
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